Methods. A radiolabeled cDNA probe of a 1(1) chain was used in slot-blot hybridization to determine the levels of a 1(1) mRNA in alkali-burned corneas. In situ hybridization was used to identify the cell types that express the a 1(1) chain. Antibodies against collagen I, a-SMA, and vimentin were used in immunohistochemical studies to determine the tissue distribution of collagen I and to identi...

Transforming growth factor (TGF) beta2 gene expression was examined in murine, rat and human lung by in situ hybridization with riboprobes. Hybridization signal was observed in a variety of cells with the sense probe, and Northern-blot analysis with this probe demonstrated the presence of a novel 3.5 kb transcript. This first report suggesting the existance of a natural TGFbeta2 antisense trans...

A dot blot hybridization immunoenzymatic assay for the rapid detection of cytomegalovirus DNA in urine samples was developed by using a digoxigenin-labeled probe which was immunoenzymatically visualized by antidigoxigenin Fab fragments labeled with alkaline phosphatase. A total of 516 urine samples from different groups of subjects were analyzed, and the hybridization assay was able to yield re...

Two 16S rRNA-targeted oligonucleotide probes were designed for the archaeal kingdoms Euryachaeota and Crenarchaeota. Probe specificities were evaluated by nonradioactive dot blot hybridization against selected reference organisms. The successful application of fluorescent-probe derivatives for whole-cell hybridization required organism-specific optimizations of fixation and hybridization condit...

Aspartic proteases are a relatively small group of enzymes which express in various nematodes including Onchocerca volvulus. An estimation of the gene copy number corresponding to the OV7A clone, which contains a cDNA insert encoding approximately two-thirds of the entire coding sequence of aspartic protease of O. volvulus, was made by slot blot analysis in a closely related species O. gibsonig...

The quick blot protocol is an improved technique for preparing crude insect homogenates for hybridization to nucleic acid probes. Individual insects are ground in wells of a microtiter plate and transferred to a dot blot manifold. This allows preparation of multiple filters and provides uniformity and an orderly arrangement of samples. The high background detection resulting from use of crude i...

Western Blot tekniği, herhangi bir örnekten istenen tek proteini özgün şekilde saptamak için moleküler biyolojide kullanılan eski ve güvenilir yöntemdir. Proteinlerin uyaranlara karşı verdiği yanıtların araştırılmasında, protein ifadesinin var-yok ya da artmış-azalmış olarak değerlendirilmesinde, hücre spesifik izoformlarının belirlenmesinde, genetik hastalıkların ortaya çıkışından sorumlu prot...