BACKGROUND Monosomic alien addition lines (MAALs) can easily induce structural variation of chromosomes and have been used in crop breeding; however, it is unclear whether MAALs will induce drastic genetic and epigenetic alterations. METHODOLOGY/PRINCIPAL FINDINGS In the present study, wheat-rye 2R and 5R MAALs together with their selfed progeny and parental common wheat were investigated thr...

The objectives of this study were to assign both microsatellite and gene-based markers on porcine chromosome X to two radiation hybrid (RH) panels and to develop a more extensive integrated map of SSC-X. Thirty-five microsatellite and 20 gene-based markers were assigned to T43RH, and 16 previously unreported microsatellite and 15 gene-based markers were added to IMpRH map. Of these, 30 microsat...

Poplars are extensively cultivated worldwide, and their susceptibility to the foliar rust fungus leads to considerable damages in plantations. To better understand the molecular basis of poplar responses to the foliar rust, suppression subtractive hybridization was used to identify the potential important or novel genes involved in the Populus szechuanica infection by Melampsora larici-populina...

A collection of 5006 full-length (FL) cDNA sequences was developed in barley. Fifteen mRNA samples from various organs and treatments were pooled to develop a cDNA library using the CAP trapper method. More than 60% of the clones were confirmed to have complete coding sequences, based on comparison with rice amino acid and UniProt sequences. Blastn homologies (E<1E-5) to rice genes and Arabidop...

Toll like receptor (TLR) signalling pathway plays a central role in inflammatory responses through the recognition of PAMPs and DAMPs. Toll-like 4 (TLR4) is well known for LPS gram-negative bacteria leading to production cytokines. Modulation TLR gene different tissues indicates functional importance innate immunity. The aim this study was clone characterize TLR4 evaluate its constitutive tissu...

Plum pox virus (PPV) Türkiye’de ilk olarak 1968 yılında Edirne ilinde tespitinden sonra birçok meyve bahçesinde saptanmıştır. Ülkesel keşif ve takip sürveyleri kapsamında PPV’nin varlığının araştırılması için Zonguldak 2019-2021 yılları arasında toplam 2539 örnek toplanmıştır. Toplanan örnekler içinde aynı ev bahçesine ait 6 adet erik ağacının yapılan DAS-ELISA RT-PCR sonucunda PPV ile enfektel...

Gastrodia pubilabiata is a nonphotosynthetic and mycoheterotrophic orchid belonging to subfamily Epidendroideae. Compared other typical angiosperm species, the plastome of G. dramatically reduced in size only 30,698 base pairs (bp). This reduction has led loss most photosynthesis-related genes some housekeeping plastome, which now contains 19 protein coding genes, three tRNAs, rRNAs. In contras...

A natural male sterile mutant of Salvia miltiorrhiza (Labiatae, Sh-B) was found during field survey in 2002. Our objective was to analyze its genetic mechanism for producing F1 hybrid seeds and to develop a molecular marker linked to male sterile gene for selection of a hybrid parent line. The segregation ratios of male sterile plants to fertile plants in the progenies of both testcross and bac...

Polyploidization contributes to the complexity of gene expression, resulting in numerous related but different transcripts. This study explored the transcriptome diversity and complexity of the tetraploid Arabica coffee (Coffea arabica) bean. Long-read sequencing (LRS) by Pacbio Isoform sequencing (Iso-seq) was used to obtain full-length transcripts without the difficulty and uncertainty of ass...

A trimeric complex formed by Tub4p, the budding yeast gamma-tubulin, and the two spindle pole body components, Spc98p and Spc97p, has recently been characterized in Saccharomyces cerevisiae. We reasoned that crucial functions, such as the control of microtubule nucleation, could be maintained among divergent species. SPC98-related sequences were searched in dbEST using the BLASTN program. Prime...