Interest in the redox properties of natural products has led to the development of various assays for the detection of antioxidant activities and ROS-scavenging properties. Here, additional modifications of the 2-deoxy-d-ribose degradation assay are introduced that specifically allow the determination of interactions of the test compound with the autoxidation of ascorbic acid and the autoxidati...

haptoglobin (hp) is a mammalian serum glycoprotein showing a genetic polymorphism with three types, 1-1, 2-2 and 1-2. hp appears to conserve the recycling of heme-iron by forming an essentially irreversible but non-covalent complex with hemoglobin which is released into the plasma by erythrocyte lysis. as an important consequence, haptoglobin-hemoglobin complex (hp-hb) shows considerable antiox...

A membrane-associated iron-binding complex was isolated from rat intestinal mucosa during iron absorption. Two 59Fe peaks (peaks 1 and 2) were separated on Sepharose 6B gel filtration from detergent solubilized 20,000 x g precipitate of upper intestinal mucosal homogenate after administration of 59Fe-labelled ferrous materials. Peak 1 was a membrane iron-binding complex whose apparent molecular...

a-Ketoglutarate-dependent enzymes catalyze many important biological oxidation/oxygenation reactions. Iron(IV)–oxo intermediates have been established as key oxidants in these oxidation reactions. While most reported model iron(II)–a-keto acid complexes exhibit stoichiometric reactivity, selective oxidation of substrates with dioxygen catalyzed by biomimetic iron(II)–a-keto acid complexes remai...

The clinically approved cardioprotective agent dexrazoxane (ICRF-187) and two of its hydrolyzed metabolites (a one-ring open form of dexrazoxane and ADR-925) were examined for their ability to protect neonatal rat cardiac myocytes from doxorubicin-induced damage. Dexrazoxane may protect against doxorubicin-induced damage to myocytes through its strongly metal-chelating hydrolysis product ADR-92...

The iron-sulfur protein of the cytochrome bc1 complex is one of a small number of proteins that are processed in two sequential steps by matrix processing peptidase (MPP) and mitochondrial intermediate peptidase (MIP) during import into Saccharomyces cerevisiae mitochondria. To test whether two-step processing is necessary for import and assembly of the iron-sulfur protein into the cytochrome b...

The behavior of rat transferrin has been investigated employing acrylamide gel electrophoresis and isoelectric focusing. In vitro trace labeling with iron chelates at 30 min was 93%-98% effective, whereas binding by simple ferric salts was reduced to 71%-76%. Complete and specific binding of 59FeSO4 by the iron binding sites of transferrin was demonstrated after in vitro or in vivo addition of ...

Over a period of sixteen months, twenty seven patients of iron deficiency anemia, who had failed to respond to treatment with various iron preparations containing iron polymaltose complex were encountered. Despite several reports of ineffectiveness or poor effectiveness of iron polymaltose, large number of iron preparations containing iron polymaltose are vigorously promoted by many pharmaceuti...

The Fe3O4@SiO2@APTMS@Glu-His@V complex was prepared with modification of iron oxide magnetic nanoparticles with (3-aminopropyl) trimethoxysilane (APTMS) and glutaraldehyde-L-histidine Schiff base followed by complexation with VOSO4. Characterization of the Fe3O4@SiO2@APTMS@Glu-His@V complex was carried out by means of FTIR, XRD, SEM, EDX, TEM, AAS and VSM techniques. It was found that Fe3O4@SiO...

The mechanisms of photodegradation of binary iron- and uranium-citrate and ternary iron-uranium-citrate complexes were elucidated. Citric acid degradation products were identified by HPLC and GC, and the metal precipitates were identified by XRD and EXAFS. Photodegradation of a binuclear iron-citrate complex occurred as a result of two one-electron oxidations of citric acid with the formation o...