BACKGROUND Early detection would be one of the most effective means to improve the outcome of renal cell carcinoma (RCC). We searched for a new plasma marker for RCC using a label-free quantitative shotgun proteomics method. METHODS Plasma proteins were digested by trypsin, and the resulting peptides were analyzed by 2-Dimensional Image Converted Analysis of Liquid chromatography mass spectro...

The importance of imaging biomarkers in biomedical research and drug design is well-acknowledged in the literature, calling for appropriate standards and guidelines for imaging biomarker development, validation and qualification. The objective of this work is to define explicitly, by means of an ontology, the vocabulary pertaining to imaging biomarkers’ domain.

The establishment of high-throughput technologies has brought substantial advances to our understanding of the biology of many diseases at the molecular level and increasing expectations on the development of innovative molecularly targeted treatments and molecular biomarkers or diagnostic tests in the context of clinical studies. In this review article, we position the two critical statistical...

Background: Despite recent advances in clinical management of laryngeal squamous cell carcinoma (LSCC), the overall 5-year survival continues to be poor. Consequently, biomarkers of treatment response will need to be identified. Proteomic strategies are one way to attempt to identify such biomarkers. Methods: The Medline, Embase and Cochrane Library databases were systematically searched until ...

Digital pathology offers powerful tools for biomarker discovery, analysis, and translation. Despite its advantages, the clinical adoption of digital has been slow. A methodological validation is required novel pathological biomarkers. Four steps are to validate a use: sample collection processing, analytical validation, utility. The biomarkers their diagnostic, monitoring, pharmacodynamic respo...

Although therapy directed against VEGF has produced clinical benefit, an understanding of responsive tumor characteristics is lacking. Blood vessel location relative to tumor cells and stromal factors may influence tumor susceptibility to VEGF-targeted therapy. Clinical validation of this potential biomarker is needed to influence clinical practice.

Downloa development of molecular markers is crucial for improving therapy in advanced non-small cell ancer (NSCLC). Here, we review perspectives of biomarker-driven personalized therapy in NSCLC, articular reference to the detection of EML4-ALK gene rearrangements. Different molecular assays, with p validation, and clinical application to different types of tissue specimens are discussed. Clin ...

for ovarian cancer testing. We will continue to post all raw data, the exact methods, and the results of the blinded trial(s) for public scrutiny. These are very important times for biomarker discovery and validation. In keeping with the National Cancer Institute Director’s 2015 challenge, effective biomarkers for early detection, risk stratification, and monitoring can save lives and reduce su...

Due to insufficient biomarker validation and poor performances in diagnostic assays, the candidate biomarker verification process has to be improved. Multi-analyte immunoassays are the tool of choice for the identification and detailed validation of protein biomarkers in serum. The process of identification and validation of serum biomarkers, as well as their implementation in diagnostic routin...

PURPOSE Medulloblastoma is the most common malignant brain tumor occurring in childhood and is a significant cause of morbidity and mortality in pediatric oncology. More intense treatment strategies are recommended for patients displaying high-risk factors; however, considerable variation in outcome remains, indicating a need for improved predictive markers. In this study, 1H magnetic resonance...