Abstract In Pseudomonas aeruginosa , Ttg2D is the soluble periplasmic phospholipid-binding component of an ABC transport system thought to be involved in maintaining asymmetry outer membrane. Here we use crystallographic structure at 2.5 Å resolution reveal that this protein can accommodate four acyl chains. Analysis available structures orthologs shows they conform a new substrate-binding-prot...

Abstract The pandemic of Coronavirus Disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome 2 (SARS-CoV-2). viral fusion protein, spike binds to its receptor Angiotensin-Converting Enzyme (ACE2) enter host cells. Further, the S1 subunit protein may be cleaved off virus and affect various organs. In addition tightly binding ACE2, also enhances peptidase activity ACE2 activate cel...

  Background:The dynamic binding capacity (DBC) of a chromatography matrix in protein purification is the amount of the total protein absorbed into the matrix, before occurrence of a significant break in the breakthrough curve. Optimization of the process criteria for maximum DBC avoids extra process scale-up and reduces ...

the surface parameter of nanoparticles such as hydrophobicity and a hydrophilicity on protein structure and function is very important. in this study, conformational changes of glucose oxidase (gox) in the mercaptopurine: gnps and 11-mercaptoundecanoic acid: gnps as a hydrophobic and a hydrophilic gnps surface was investigated by various spectroscopic techniques, including: uv-vis absorption, f...

protein structural flexibility is important for catalysis, binding, protein design, and allostery. some simple methods have recently been introduced to compute protein flexibility directly from the protein structure without any mechanical models. for example the atomic mean square displacement (or b-factor) is related to the number of neighboring atoms. the protein structure can be modeled as a...

The interaction between [Pt(phen)(pyrr-dtc)]NO3 (where phen = 1,10-phenanthroline and pyrr-dtc =pyrrolidinedithiocarbamat) with human serum albumin (HSA) was studied by fluorescence, UV–vis absorption, circular dichroism (CD) spectroscopy and molecular docking technique under like physiological condition in Tris–HCl buffer solution at pH 7.4. UV-Vis absorption spectroscopy indicates that the pro...

to obtain drugs which are more selective at benzodiazepine (bzd) receptors, design and synthesis of functionally selective ligands for bzd receptors is the current strategy of our pharmaceutical chemistry department. the affinity of newly synthesized ligands is assessed by radioligand receptor binding assays. based on our previous studies, 2-phenyl-5-oxo-7-methyl-1,3,4-oxadiazolo[a,2,3]-pyrimid...

curcumin is a natural polyphenolic compound with anti-cancer, anti-inflammatory, and anti-oxidation properties. low water solubility and rapid hydrolytic degradation are two challenges limiting use of curcumin as therapeutic agent. in the current study, the role of the native/modified forms of bovine serum albumin (bsa) and casein, as food-grade biopolymers and safe drug delivery systems, on th...

Orange Carotenoid Protein (OCP) is a ketocarotenoid-binding protein essential for photoprotec- tion in cyanobacteria. The main steps of the photoactivated conversion which converts OCP from its resting state to the...