Understanding of cellular processes and underlying molecular events requires knowledge about different aspects of molecular interactions, networks of molecules and pathways in addition to the sequence, structure and function of individual molecules involved. Databases of interacting molecules, pathways and related chemical reaction equations have been developed. The kinetic data for these inter...

The specific binding of [3H]phorbol 12,13-dibutyrate ([3H]PDBU) to participate preparations from mouse brain has been further characterized. Kinetic analysis, using a filtration assay to measure binding, yielded a second-order rate constant at 23°of 3.75 x 107 M~' min"1 and a first-order dissociation rate constant of 0.21 min~1. The Kd of 5.6 nw calculated from the kinetic data agreed well wit...

Glycoluril derivatives with a carboxylic acid side chain have been synthesized and shown to bind to both avidin and streptavidin. Introduction of a valerate side chain in glycoluril led to an increased binding to both proteins only when the valerate group was bound to a N atom and with the proper stereochemistry [(+)-enantiomer]. On the other hand, introduction of the valerate side chain either...

The specific binding of human granulocyte colony-stimulating factor (G-CSF) to its receptors on NFS-60 cells acts as a primer for cellular proliferation. There are approximately 400 binding sites per cell. with a binding constant of about 1 00 pmol/ 1. Before the proliferative response, the affinity constant of the membrane particulate fraction to labeled guanosine triphosphate-gamma-S ( ‘S-GTP...

The specific binding of human granulocyte colony-stimulating factor (G-CSF) to its receptors on NFS-60 cells acts as a primer for cellular proliferation. There are approximately 400 binding sites per cell. with a binding constant of about 1 00 pmol/ 1. Before the proliferative response, the affinity constant of the membrane particulate fraction to labeled guanosine triphosphate-gamma-S ( ‘S-GTP...

A new chromatographic method was developed for characterizing allosteric interactions between an immobilized binding agent and low-solubility compounds. This approach was illustrated by using it to characterize the interactions between tamoxifen and warfarin during their binding to the protein human serum albumin (HSA), with beta-cyclodextrin being employed as a solubilizing agent for these dru...

The interaction between propranolol (PROP) and human serum albumin (HSA) was studied in the presence of dimethyl methylphosphonate (DMMP). DMMP is usually considered as a simulant for chemical warfare agents (CWAs). For this purpose fluorescence quenching, resonance light scattering (RLS), synchronous, three-dimensional fluorescence spectroscopy and molecular dynamics (MD) simulation were emplo...