Fluorescence emission spectroscopy was used to investigate interactions between two effectors and BenM, a transcriptional regulator of benzoate catabolism. BenM had a higher affinity for cis,cis-muconate than for benzoate as the sole effector. However, the presence of benzoate increased the apparent dissociation constant (reduced the affinity) of the protein for cis,cis-muconate. Similar result...

Two genes of the meta pathway of phenol degradation were cloned from a phenol-utilizing strain of Bacillus stearothermophilus and were mapped by subcloning and by use of a Tn5 insertion mutation. They code for phenol hydroxylase and catechol 2,3-dioxygenase, respectively. The gene encoding catechol 2,3-dioxygenase, which is more thermostable than catechol 2,3-dioxygenase encoded by the other ge...

Biphenyl is a compound in which two benzene rings are connected to each other. Polychlorinated biphenyls (PCBs) can be produced by the direct chlorination of biphenyl, by which 209 different compounds containing 1 to 10 chlorines can be produced. Because PCBs have been widely used for a variety of industrial purposes, these recalcitrant compounds are recognized to be some of the most serious en...

Cupriavidus pinatubonensis JMP134, like many other environmental bacteria, uses a range of aromatic compounds as carbon sources. Previous reports have shown a preference for benzoate when this bacterium grows on binary mixtures composed of this aromatic compound and 4-hydroxybenzoate or phenol. However, this observation has not been extended to other aromatic mixtures resembling a more archetyp...

The objective of this study was to determine the stability of metronidazole benzoate suspension in SyrSpend SF One-Step Suspension system. The studied samples were packaged in 60-mL amber plastic prescription bottles, which were stored protected from light under controlled environmental conditions for a period of 360 days. Stability of metronidazole benzoate suspension in SyrSpend SF was assess...

ABSTRACT RNA-seq analysis of Cupriavidus necator NH9, a 3-chlorobenzoate degradative bacterium, cultured with 3-chlorobenzaote and benzoate, revealed strong induction genes encoding enzymes in degradation pathways the respective compound, including to convert benzoate chlorocatechol catechol, respectively, ortho-cleavage pathway for conversion central metabolites. The transporters, components s...

sodium benzoate and potassium sorbate are two major chemical preservatives which are used in doogh (iranian traditional dairy drink). in this study, a total of 27 commercial brands of highly consumed of doogh samples were analyzed. the means and standard deviation for concentration of these preservatives based on hplc results for analysis of benzoate and sorbate were 195·9 (sd 1·8) and 328·8 (s...

A flavoprotein, salicylate hydroxylase (salicylate, DPNH : oxygen oxidoreductase (I -hydroxylation, l-decarboxylating)), has been induced and isolated from a soil bacterium grown on salicylate as sole carbon source. In contrast to a similar enzyme studied by YAMAMOTO, S., KATAGIRI, M., MAENO, H., AND HAYAISHI, 0. ((1965) J. Bid, Chem. 240, 3408) this enzyme is dimeric with two subunits and two ...

Tryptophan degradation in mice initiated by indoleamine 2,3-dioxygenase was characterized, taking advantage of its induction by bacterial lipopolysaccharide. Our results demonstrated that in various tissues, N-formylkynurenine produced by the dioxygenase from tryptophan was rapidly hydrolyzed into kynurenine by a kynurenine formamidase, but it was not further metabolized. The localization in th...

There are only two known thiol dioxygenase activities in mammals, and they are ascribed to the enzymes cysteine dioxygenase (CDO) and cysteamine (2-aminoethanethiol) dioxygenase (ADO). Although many studies have been dedicated to CDO, resulting in the identification of its gene and even characterization of the tertiary structure of the protein, relatively little is known about cysteamine dioxyg...