The endosymbiont Wolbachia wAlbB induces refractoriness to Plasmodium falciparum in Anopheles stephensi, the primary mosquito vector of human malaria in the Middle East and South Asia. However, it remains unknown whether such refractoriness can be extended to other malaria species. In particular, it was reported that under very specific conditions, wAlbB can enhance Plasmodium infection in some...

Anopheles melas is a brackish water mosquito found in coastal West Africa where it is a dominant malaria vector locally. In order to facilitate genetic studies of this species, 45 microsatellite loci originally developed for Anopheles gambiae were sequenced in An. melas. Those that were suitable based on repeat number and flanking regions were examined in 2 natural populations from Equatorial G...

Mosquitoes are blood-feeding insects and serve as the most important vectors for spreading human diseases such as malaria, yellow fever, dengue fever, and filariasis. The continued use of synthetic insecticides has resulted in resistance in mosquitoes. Synthetic insecticides are toxic and affect the environment by contaminating soil, water, and air, and then natural products may be an alternati...

BACKGROUND Mosquitoes transmit serious human diseases, causing millions of death every year. Vector control is facing a threat due to the emergence of resistance to synthetic insecticides. Insecticides of botanical origin may serve as suitable alternative biocontrol techniques in the future. Nine different locally available medicinally important plants suspected to posse larvicidal property wer...

In this study, we synthesized and characterized (UV–vis, XRD, FTIR, SEM, TEM, AFM) Trewia nudiflora-assisted silver nanoparticles (Tn-AgNPs). The toxicity of T. nudiflora leaf aqueous extract Tn-AgNPs was tested against Anopheles stephensi, Aedes aegypti, Culex quinquefasciatus larvae its LC50 values were 121.82/16.69, 130.51/18.11, 142.38/19.53 μg mL–1, respectively. Moreover, the biotoxicity ...

Heritable RNA interference (RNAi), triggered from stably expressed transgenes with an inverted repeat (IR) configuration, is an important tool for reverse genetic studies. Here we report on the development of stable RNAi in Anopheles stephensi mosquitoes, the major vector of human malaria in Asia. Trans genic mosquitoes stably expressing a RNAi transgene, designed to produce intron-spliced doub...

BACKGROUND One of the best ways to control the malaria disease and to be protected human against Anopheles mosquito biting is the use of repellents. Throughout repellents, herbal ones may be an appropriate and safe source for protection. METHODS Chemical constituents of Achillea vermiculata and Satoreja hortensis were determined by using gas chromatography-mass spectrometry. Efficacy and the ...