For a commercially viable recombinant intracellular protein production process, efficient cell lysis and protein release is a major bottleneck. The recovery of recombinant protein, cholesterol oxidase (COD) was studied in a continuous bead milling process. A full factorial response surface methodology (RSM) design was employed and compared to artificial neural networks coupled with genetic algo...

Isolation and purification of biomolecules (doubleand singlestranded DNA, total RNAs, mRNAs, miRNA, proteins), as well as specific cells and organs, facilitate a wide array of downstream applications in gene and protein expression studies, cloning, transfection, protein-protein interactions, immunology, clinical diagnostics, cDNA library synthesis, PCR and qPCR, Sanger and NGS sequencing, and m...

Based on extensive Brownian dynamics simulations we study the thermally driven motion of a tracer bead in a cross-linked, dynamic gel network in the limit when the tracer bead’s size is of the same size or even larger than the equilibrium mesh size of the gel. The analysis of long individual trajectories of the tracer bead demonstrates the existence of pronounced transient anomalous diffusion, ...

The influence of the bead-bead interaction on the rotational dynamics of macromolecules which are immersed into a solution has been investigated by starting from the microscopic theory of the macromolecular motion, i.e., from a Fokker-Planck equation for the phase-space distribution function. From this equation, we then derived an explicit expression for the configuration-space distribution fun...

Proper fusion is crucial in generating a sound weld. Successful control of the fusion state requires accurate measurements of both the top-side and back-side bead widths. A top-side sensor based system is preferred so that the sensor can be attached to and moved with the torch. Thus, the system must be capable of estimating the back-side bead width with high accuracy. Because skilled human oper...

VASP comet sightings ess is more when it comes to actin-fueled propulsion. Julie Plastino, Stéphane Olivier, and Cécile Sykes (Institut Curie, Paris, France) show that hollow actin comets propel beads faster than a more filament-packed comet. Actin comets, which drive bead or bacteria movement, are thought to depend strongly on Arp2/3–built, branched actin networks. Now, Plastino et al. show th...

The effect of process parameters on the forming profile nickel-based alloy deposited by plasma and cold metal transfer (CMT) wire-arc deposition was comparatively studied. arc welding (PAW) presented good wettability high dilution rate, while CMT showed opposite. ellipse parabola function models for single weld bead were established MATLAB software. results that model had higher similarity with...

User modeling approaches are expected to fulfill user modeling criteria. We present a collection of such criteria, and analyze how the info-bead user modeling approach fulfills them. The criteria are all well-known criteria elicited from the user modeling literature, including Kay [1999], Fink and Kobsa [2000], Kobsa [2001; 2007], and Vassileva [2003]. We arranged the criteria according to the ...

This study proposes a method for determining the near-optimal settings of welding process parameters using a controlled random search (CRS) wherein the near-optimal settings of the welding process parameters are determined through experiments. The method suggested in this study is used to determine the welding process parameters by which the desired weld bead geometry is formed in gas metal arc...

We present a new method for calibrating an optical-tweezer setup that does not depend on input parameters and is less affected by systematic errors like drift of the setup. It is based on an inference approach that uses Bayesian probability to infer the diffusion coefficient and the potential felt by a bead trapped in an optical or magnetic trap. It exploits a much larger amount of the informat...