One of the major obstacles of Bt production as a biopesticide is its expensive bioprocess and fermentation. Therefore, the objective of the present study was to optimize growth condition and develop a low-cost bioprocess for mass production of a native coleopteraneffective Bacillus thuringiensis (Bt) strain (KH4) based on agricultural wastes, at incubator and batch fermenter level. Preliminary ...

BACKGROUND Monascus mycelia and pigments are promising sources of food and medicine with their potential pharmaceutical values and health-improving functions. Using high cell density fermentation of Monascus spp. to achieve higher mycelium and yellow pigment production is worthy to be researched. In this study, the characteristics and productivity shifting of pigments in high cell density cultu...

The principal objective of this study was to evaluate the kinetics of dihydroxyacetone production by Gluconobacter frateurii CGMCC 5397 under different oxygen volumetric mass transfer coefficient (kLa) conditions in submerged bioreactors using biodiesel-derived crude glycerol as the carbon source. kLa is a key fermentation parameter for the production of dihydroxyacetone. Cultivations were cond...

Animal waste fats were explored as a fermentation substrate for the production of high-value unsaturated single cell oil (SCO) using oleaginous fungi, Mucor circinelloides and Mortierella alpina. Both strains showed good growth lipid accumulation when animal fat carbon source. The biomass concentration 16.7 ± 2.2 gDCW/L content 54.1%wt (of dry weight) obtained in shake flask experiments, surpas...

The traditional CaCO3-based fermentation process generates huge amount of insoluble CaSO4 waste. To solve this problem, we have developed an efficient and green D-lactic acid fermentation process by using ammonia as neutralizer. The 106.7 g/L of D-lactic acid production and 0.89 g per g of consumed sugar were obtained by Sporolactobacillus inulinus CASD with a high optical purity of 99.7% by ad...

The production of lipids by oleaginous yeast and fungi becomes more important because these lipids can be used for biodiesel production. To understand the process of lipid production better, we developed a model for growth, lipid production and lipid turnover in submerged batch fermentation. This model describes three subsequent phases: exponential growth when both a C-source and an N-source ar...

The fermentation kinetics of L-glutamic acid by Corynebacterium glutamicum was studied in a batch bioreactor. Mathematical model using the logistic equation for growth, Leudeking-Piret kinetic equation for product formation and Leudeking-Piret like equation for substrate consumption was proposed. Based on the analysis of experimental data followed by computer simulation, the model seemed to pro...

Using dark fermentation, hydrogen can be generated from renewable organics including waste materials. Key to successful application of anaerobic fermentation is to uncouple liquid and biomass retention times in reactor system. This paper reviews reactor configurations (fixed-bed, fluidized-bed, upflow anaerobic sludge blanket and continuous stirred tank reactors) and operating processes (batch,...

batch Fermentation by Means of Genetic Programming K.D. BETTENHAUSEN , P. MARENBACH , S. FREYER , H. RETTENMAIER and U. NIEKEN Darmstadt University of Technology , Institute of Control Engineering, Department of Control Systems Theory & Robotics, Landgraf-Georg-Strasse 4, D-64283 Darmstadt, Germany BASF AG, D-67056 Ludwigshafen, Germany Abstract. The article at hand describes an approach for th...

In this work, Evolutionary Algorithms (EAs) are used to control a recombinant bacterial fed-batch fermentation process, that aims to produce a bio-pharmaceutical product. Initially, a novel EA was used to optimize the process, prior to its run, by simultaneously adjusting the feeding trajectory, the duration of the fermentation and the initial conditions of the process. Finally, dynamic optimiz...