Each Bartonella species appears to be highly adapted to one or a limited number of reservoir hosts, in which it establishes long-lasting intraerythrocytic bacteremia as the hallmark of infection. Recently, we identified Trw as the bacterial system involved in recognition of erythrocytes according to their animal origin. The T4SS Trw is characterized by a multiprotein complex that spans the inne...

The hemagglutinin-neuraminidase (HN) protein of Newcastle disease virus (NDV) with its immunotherapeutic activities and sialic acid binding abilities is a promising cancer adjuvant. The HN was surfaced displayed on Lactococcus lactis and its cancer targeting ability was investigated via attachment to the MDA-MB231 breast cancers. To surface display the HN protein on the bacterial cell wall, HN ...

A robust bacterial display methodology was developed that allows the rapid isolation of peptides that bind to arbitrarily selected targets with high affinity. To demonstrate the utility of this approach, a large library (5 x 10(10) clones) was constructed composed of random 15-mer peptide insertions constrained within a flexible, surface exposed loop of the Escherichia coli outer membrane prote...

Abstract Bacterial cis -epoxysuccinic acid hydrolases (CESHs) are intracellular enzymes used in the industrial production of enantiomeric tartaric acids. The mainly as whole-cell catalysts because low stability purified CESHs. However, cell permeability is major drawback catalyst. To overcome this problem, we developed using various surface display systems for CESH[L] which produces L(+)-tartar...

We present the construction and screening of yeast display libraries post-translationally modified peptides wherein site-selective enzymatic treatment linear is achieved using bacterial transglutaminase. To this end, we developed two alternative routes, namely (i) followed by with recombinant transglutaminase in solution; or (ii) intracellular co-expression to achieve peptide modification endop...

Surface layer as the outer protective coverage of bacteria and archaea are two-dimensional crystalline and symmetrical arrays of proteins that recently attract a lot of attention for biologist scientists. The surface layers of bacteria are usually 5 to 10 nm in diameter and represent highly porous protein lattices with uniform size and morphology with the pore sizes of 2 to 8 nm. The crucial an...