Introduction: Disseminated cryptococcosis mainly occurs in patients with impaired cell mediated immunity. Case Presentation: We present a case of disseminated cryptococcosis, in a non-HIV patient with steroid resistant nephrotic syndrome (focal segmental glomerulosclerosis), who was given various immunosuppressive agents. Discussion: BACTEC blood culture, cerebrospinal fluid analysis, and chest...

Prosthetic joint infection (PJI) is a rare but refractory complication of arthroplasty. Accurate identification of pathogens is a key step for successful treatment of PJI, which remains a challenge for clinicians and laboratory workers. We designed a combined culture method with sonication of implants and incubation in a BD Bactec system to improve the effectiveness of pathogen diagnosis in PJI...

Introduction: A comparative study was conducted to identify the genus and species of non-fermenting gram-negative bacilli isolated from blood culture samples using phenotypic methods Phoenix method. Objectives: This aimed evaluate system compared currently available process. Materials Methods: descriptive-analytical cross-sectional evaluated 30 collected 2019 2020 patients at AL Zahra Kashani h...

Introduction: This study compared phenotypic methods and the 16S rRNA gene sequencing method to identify genus species of non-fermenting gram-negative bacilli isolated from blood culture samples. Objectives: The aim this was evaluate effectiveness using in detecting non-fermented bacteria Materials Methods: A cross-sectional conducted April 2019 2020, including all patients who required sterile...

Objective: An irreversible process begins when a systemic infection causes sepsis. Therefore, rapid identification of the agent bacteria in sepsis and its antibiotic resistance is crucially important. In this study, it was aimed to investigate efficiency genotype test.
 Methods: Blood culture bottles sent laboratory were examined with an automatic blood system (BACTEC, BD, USA). Growing id...

When 24 different aerobic organisms were grown in a shaken culture, all were found to first absorb gas from the headspace. In a rudimentary medium, such as tryptic soy broth, 16 of the 24 organisms did not produce gas following the initial gas absorption. We have developed a simple, noninvasive method for detecting both gas absorption and production in multiple culture vials. The time to positi...

Background & Aims: Brucellosis is a public health problem in many developing countries, including Iran. Diagnosis is based on history, clinical manifestations and positive serum agglutination test (SAT) or Wright test. The purpose of this study was to evaluate the diagnostic value of SAT in pediatric Brucellosis. Methods: In this prospective study, during 2 years, 52 patients with clinical feat...

A prospective study of 111 adult patients with a pleural effusion was carried out in an area with a high prevalence of tuberculosis to compare the yield of bedside with laboratory inoculation of pleural fluid, and the yield and speed of a radiometric mycobacterial culture system (BACTEC) with that of conventional culture. The use of adenosine deaminase activity in pleural fluid as a diagnostic ...