AIM To characterize AmpC-beta lactamases among Enterobacteriaceae isolates from clinical samples at Mbarara Regional Referral Hospital. STUDY DESIGN Laboratory-based descriptive cross-sectional study. PLACE AND DURATION OF STUDY Microbiology Department, Mbarara Regional Referral Hospital and MBN clinical Laboratories, between May to September 2013. METHODOLOGY This study included 293 Ente...

In a UK survey of the occurrence of extended spectrum beta-lactamases, 96 hospitals submitted a total of 3951 non-selected, non-duplicate isolates of Enterobacteriaceae from 100 patients in each hospital, 206 of these cultures being mixed and, therefore, discarded. These isolates were initially screened for strains likely to produce extended-spectrum beta-lactamases (ESBLs) by MIC determination...

This manual provides guidance for reporting antimicrobial resistance under the framework of Directive 2003/99/EC and Commission Implementing Decision (EU) 2020/1729 in food-producing animals foodstuffs derived thereof. The objective is to harmonise streamline made by Member States ensure that data collected are relevant easy analyse at European Union level. Detailed guidelines provided text for...

Escherichia coli (E. coli) is a zoonotic bacterial pathogen commonly found in human, birds and animals. The widespread use of broad-spectrum beta-lactams for poultry treatment, has resulted the production extended spectrum β-lactamases (ESBLs) E. coli. In this study, we analyzed antimicrobial resistance pattern isolates from broiler chickens ducks with clinical colibacillosis, investigated pres...

The emergence of antimicrobial resistance in Klebsiella spp., including resistance to extended-spectrum cephalosporins (ESC) and fluoroquinolones, is of great concern in both human and veterinary medicine. In this study, we investigated the prevalence of antimicrobial resistance in a total of 103 Klebsiella spp. isolates, consisting of Klebsiella pneumoniae complex (KP, n = 89) and K. oxytoca (...

The modified Hodge method (MHT) has been recommended by the CLSI for confirmation of suspected class A carbapenemase production in species of Enterobacteriaceae. This test and the Masuda method (MAS) have advantages over traditional phenotypic methods in that they directly analyze carbapenemase activity. In order to identify the potential interferences of these tests, we designed a panel compos...

Salmonella spp. producing extended-spectrum beta-lactamases (ESBLs) have been reported in many countries, but there is no information on their prevalence in Africa. ESBL-producing Salmonella enterica serotype Isangi and S. enterica serotype Typhimurium strains have been noted in South Africa since 2001. A total of 160 consecutive isolates of Salmonella spp. were collected from 13 hospitals loca...

conclusions a high prevalence of multiple β-lactamase production was observed among the ampc producers (60%), of which the majority co-produced ampc and mbl. the current study results showed correlation between β-lactamase production and the presence of antibiotic resistance genes in the isolates. results all isolates were multidrug resistant. ampc, esbl and mbl production were observed in 35 (...

BACKGROUND In order to investigate the epidemiology, molecular characteristics, and distribution of extended-spectrum β-lactamase (ESBL)- and AmpC-producing Escherichia coli from community-onset infections in Chinese county hospitals. METHODS E. coli isolates were collected from patients with community-onset infections in 30 county hospitals. ESBL activity was confirmed by double-disc diffusi...