Thermal death rate data were obtained for spores of Clostridium sporogenes P.A. 3679 and Bacillus subtilis var. niger, and for cells of Salmonella senftenberg 775W. The survival curves for P.A. 3679 were approximately linear, but for B. subtilis var. niger or S. senftenberg 775W they were sigmoidal. Decimal reduction times were derived from the regression slopes of the apparent linear portion o...

Zamenhof, Stephen (University of California, Los Angeles), Hela Bursztyn, T. K. Ramachandra Reddy, and Patrice J. Zamenhof. Genetic factors in radiation resistance of Bacillus subtilis. J. Bacteriol. 90:108-115. 1965.-A study of several wild cross-transformable strains of Bacillus subtilis revealed differences in the resistance of their spores to X rays. Closer study of two such strains reveale...

This work was undertaken to obtain information on levels of metabolism in dormant spores of Bacillus species incubated for weeks at physiological temperatures. Spores of Bacillus megaterium and Bacillus subtilis strains were harvested shortly after release from sporangia and incubated under various conditions, and dormant spore metabolism was monitored by (31)P nuclear magnetic resonance (NMR) ...

Short exposures of Bacillus spores to nutrient germinants can commit spores to germinate when germinants are removed or their binding to the spores' nutrient germinant receptors (GRs) is inhibited. Bacillus subtilis spores were exposed to germinants for various periods, followed by germinant removal to prevent further commitment. Release of spore dipicolinic acid (DPA) was then measured by diff...

We evaluated the efficacy of a commercial washer-pasteurizer. Carriers were inoculated with 10(4) to 10(6) test organisms and pasteurized at 170 degrees F for 30 minutes. Pasteurization eliminated all test organisms with the exception of Bacillus subtilis spores. Pasteurization appears efficacious for the disinfection of respiratory-care equipment and could result in a cost savings of approxima...

This paper describes a molecular-based method which is able to discriminate between viable and inactivated Bacillus subtilis spores by utilizing the DNA-intercalating dye propidium monoazide. The approach should be valuable in our attempt to employ molecular methods to streamline the evaluation of process validation using bacterial endospores.

Esculin agar has been found to be a simple, inexpensive, rapid, and reliable means to promote production of spores of inhibitor-sensitive clones of Bacillus subtilis strains ATCC 6051 and 6633 for use in the Guthrie bioassay screening tests for genetic metabolic disorders.

Elimination of contaminating spores on packaging materials and food-contact surfaces remains a challenge to the food industry. Hydrogen peroxide and chlorine are the most commonly used sanitizers to eliminate these contaminants, and ozone was recommended recently as an alternative. Hence, we compared the sporicidal action of ozone and hydrogen peroxide against selected foodborne spores of Bacil...

The nucleotide excision repair (NER) and spore photoproduct lyase DNA repair pathways are major determinants of Bacillus subtilis spore resistance to UV radiation. We report here that a putative ultraviolet (UV) damage endonuclease encoded by ywjD confers protection to developing and dormant spores of B. subtilis against UV DNA damage. In agreement with its predicted function, a His(6)-YwjD rec...