A truncated fragment of the cycloinulo-oligosaccharide fructanotransferase (CFTase) gene of Bacillus circulans MCI-2554 was fused to the prepro secretion sequence of the alpha-factor and expressed in Saccharomyces cerevisiae under the control of the 5' upstream region of the isocitrate lyase gene of Candida tropicalis (UPR-ICL). Efficiently secreted recombinant CFTase protein (yeast CFTase) was...

ABSTRACT Late leaf spot (LLS), caused by Phaeoisariopsis personata, is a foliar disease of groundnut or peanut (Arachis hypogaea) with high economic and global importance. Antifungal and chitinolytic Bacillus circulans GRS 243 and Serratia marcescens GPS 5, selected among a collection of 393 peanut-associated bacteria, were applied as a prophylactic foliar spray and tested for control of LLS. C...

By using natural-abundance (13)C-nuclear magnetic resonance spectroscopy and high-performance liquid chromatography (HPLC) analysis we have investigated the types of compatible solutes that are synthesized de novo in a variety of Bacillus species under high-osmolality growth conditions. Five different patterns of compatible solute production were found among the 13 Bacillus species we studied. ...

The main objective of this study was to determine the effects of plant growth promoter bacteria on biomass and yield of basil. The experiment was carried out as randomized complete blocks design with eight treatments and three replications at research field of Agriculture Company of Ran in Firouzkuh of iran in 2012 . The treatments were [1] Azotobacter chroococcum (A), [2] Azospirillum lipoferu...

These experiments were conducted to study the response of some Egyptian cotton, Giza 96 (extra-long stable) and stable)varieties its yield characteristic, lint percentage, fiber yarn properties when using bio-fertilizers such as vermicompost, Bacteria  bacteria inoculums (Bacillus polymxa, Bacillus megaterium circulans) algae extract under different growing area, Sakha region (clay soil) El-Nub...

The mannanase gene of B. circulans NT 6.7 was cloned and expressed in Escerichia coli and food-grade Lactobacillus plantarum expression system. B. circulans NT 6.7 mannanase gene consisted of 1,083 nucleotides encoding 360 amino acid residues which belonging to glycosyl hydrolase family 26. In E. coli system, mannanase gene was cloned into pET21d and expressed in E. coli BL21* (DE3). Beta-manna...