The erythrocytic-stage surface protein, Equi Merozoite Antigen 1 (EMA-1), is a major candidate for the development of a diagnostic antigen for equine piroplasmosis. In order to establish an effective diagnostic method for practical use, the gene encoding the entire EMA-1 of Theileria equi Jaboticabal strain was cloned and expressed in Escherichia coli as a histidine-tagged protein (His6-EMA1). ...

Babesiosis is a tick-borne disease affecting humans and many domestic and wild animals. Domestic animals showing appreciable morbidity and mortality include dogs, cats, cattle and horses. Both canine and feline babesiosis are diseases characterised by haemolytic anaemia, icterus and haemoglobinuria. Canine babesiosis can range from chronic or subclinical to peracute and fatal, depending on the ...

Background: Babesia is blood-tissue parasite, which are transmitted by hard ticks from Ixodidae family. The parasite is the cause of Babesiosis among ruminants. Babesia is as one of main fatal factors among livestock in endemic regions such as Iran. The aim of this study was to investigate Babesia spp infection using microscopic and molecular methods among small ruminants in Mazandaran and Gole...

Equine piroplasmosis is a tick-transmitted protozoan disease caused by Theileria equi and/or Babesia caballi. In the present study, we expressed a 53kDa protein from the truncated EMA-2 gene of T. equi (Indian strain) and developed EMA-2ELISA using this expressed protein. This ELISA is able to detect T. equi-specific antibodies in experimentally infected animals as early as 9 days post-infectio...

PCR Detection of Babesia ovata from Cattle Reared in Japan and 1 Clinical Significance of Co-Infection with Theileria orientalis 2 3 Running title: Co-infection of B. ovata and T. orientalis in cattle 4 5 Thillaiampalam Sivakumar, Michihito Tagawa, Takeshi Yoshinari, Adrian P. Ybañez, 6 Ikuo Igarashi, Yuzuru Ikehara, Hiroshi Hata, Seiji Kondo, Kotaro Matsumoto, 7 Hisashi Inokuma, and Naoaki Yok...

This study was carried out to determine the presence and distribution of tick-borne haemoprotozoan parasites (Theileria and Babesia) in apparently healthy cattle in the East Black Sea Region of Turkey. A total of 389 blood samples were collected from the animals of various ages in six provinces in the region. Prevalence of infection was determined by reverse line blot (RLB) assay. The hypervari...

Antibodies that recognized either Babesia gibsoni or canine red blood cell (RBC) 70-kilodalton (kDa) protein were detected in serum from acutely and chronically B. gibsoni-infected. In those sera, antibodies that reacted with recombinant B. gibsoni and canine heat shock protein 70 (rBgHsp70 and rcHsp70) were detected; therefore, B. gibsoni and canine RBC 70-kDa proteins seemed to be BgHsp70 and...

A rapid and accurate assay for evaluating antibabesial drugs on a large scale is required for the discovery of novel chemotherapeutic agents against Babesia parasites. In the current study, we evaluated the usefulness of a fluorescence-based assay for determining the efficacies of antibabesial compounds against bovine and equine hemoparasites in in vitro cultures. Three different hematocrits (H...

This study aimed to assess the prevalence of Babesia ovis infection in adult Rhipicephalus bursa and small ruminants in West Azerbaijan province, Iran. Blood samples were collected from 280 sheep and 122 goats of forty randomly selected flocks. Specific B. ovis fragment was detected in 67 animals (16.7%), of which 52 animals (18.6%) were sheep and 15 animals (12.2%) goats (P < 0.05). Of the 848...

Background Ehrlichia, Anaplasma, and Babesia spp. are canine pathogens transmitted by the Rhipicephalus sanguineus tick which can cause varied clinical signs. These pathogens have been investigated in the Philippines, but coinfection has not been reported yet. Aim The aim of this study was to evaluate the presence of Ehrlichia/Anaplasma and Babesia spp. in Philippine dogs. Materials and Met...