BACKGROUND: Colibacillosis is one of the most prevalentdiseases in the world that causes multimillion-dollar annuallosses. OBJECTIVES: In order to evaluate molecular epidemiologyof some virulence associated factors in Escherichia coli,isolated from poultry, the presence of iut A, iss, hly F, omp T, iroN, afa, sfa (S)and pap G (II) were investigated by multiplex PCRassay. METHODS: Two hundred th...

Resistance to extended-spectrum cephalosporins (ESC) is a global health issue. The aim of this study was to analyze and compare plasmids coding for resistance to ESC isolated from 16 avian commensal and 17 avian pathogenic Escherichia coli (APEC) strains obtained respectively at slaughterhouse or from diseased broilers in 2010-2012. Plasmid DNA was used to transform E. coli DH5alpha, and the re...

The extraintestinal pathogen, avian pathogenic E. coli (APEC), known to cause systemic infections in chickens, is responsible for large economic losses in the poultry industry worldwide. In order to identify genes, involved in the early essential stages of pathogenesis, namely adhesion and colonization, a lung colonization model of infection was established in 5-week old White leghorn specific-...

Adhesion of pathogen to host cells is an important prerequisite for successful colonization and establishment of the pathogenesis. The aim of this study is to examine the function of FimH adhesin in the adherence of avian pathogenic E. coli to porcine intestinal epithelial cell lines (IPEC-J2) and human lung epithelial cell line (A549) in an in vitro infection model. Three strains of avian path...

Avian pathogenic E. coli (APEC) can cause avian colibacillosis, an economically important disease that contributes to bird mortality and the high costs associated with prevention treatment. Little is known about APEC in context of Australian conditions. The current study aimed estimate prevalence determine risk factors cloacal carriage among commercial healthy meat chickens Queensland. Cloacal ...

AIMS To isolate and characterize bacteriophages, and to evaluate its lytic performance against avian pathogenic Escherichia coli (APEC) strains with high patterns of antibiotic resistance, in order to select phages for a therapeutic product to treat colibacillosis in chickens. METHODS AND RESULTS Bacteriophages were isolated from poultry sewage and tested against 148 O-serotyped APEC strains....

Avian pathogenic Escherichia coli (APEC) is associated with extraintestinal infections in poultry causing a variety of diseases collectively known as colibacillosis. The host and bacterial factors influencing and/or responsible for carriage and systemic translocation of APEC inside the host are poorly understood. Identification of such factors could help in the understanding of its pathogenesis...

Abstract Pathogenic strains of Escherichia coli may invade the subcutaneous tissue poultry and cause cellulitis, whilst pathogen also lesions in internal organs such as liver. Current paper co-relates virulence genes characteristic Avian (APEC) broilers´ cellulitis liver lesions. One hundred carcasses were retrieved from production chain an avian abattoir state Bahia, Brazil, between August 201...

Colibacillosis accounts for annual multimillion dollar losses in the poultry industry, and control of this disease is hampered by limited understanding of the virulence mechanisms used by avian pathogenic Escherichia coli (APEC). Previous work in our laboratory has found that the presence of the increased serum survival gene (iss) is strongly associated with APEC but not commensal E. coli, maki...