The glucosinolate autolysis products of crushed seeds of DesclIrainia sophia L. (khakeshi) and Brassica napus L. (shelghem) were administered orally to rats in doses representing 8,16 and 32 gm seeds/kg body weight/day at intervals of 10, 20 and sometimes 30 days. Serum T3 and T4 concentrations were reduced in 10 day•autolysate treated animals in a similar fashion to that found with methim...

The two-component regulatory system, WalR/WalK is necessary for growth of different gram-positive bacteria, including Staphylococcus aureus. In present study, we confirmed the essentiality of both the histidine kinase protein WalK and the response regulator WalR for growth using S. aureus RN4220 strain and demonstrated that the histidine kinase protein WalK and the response regulator WalR funct...

Digestive trypsins undergo proteolytic breakdown during their transit in the human alimentary tract, which has been assumed to occur through trypsin-mediated cleavages, termed autolysis. Autolysis was also postulated to play a protective role against pancreatitis by eliminating prematurely activated intrapancreatic trypsin. However, autolysis of human cationic trypsin is very slow in vitro, whi...

The activity of matrix metalloproteinase-2 (MMP-2) is regulated stringently on the posttranslational level. MMP-2 efficiently undergoes autolysis into inactive polypeptides in vitro, prompting the hypothesis that MMP-2 autolysis may function as an alternative mechanism for posttranslational control of MMP-2 in vivo. Moreover, MMP-2 binds to intact type I collagen fibrils; however, the functiona...

Zusammenfassung: Der Beitrag zeigt, wie die von Niklas Luhmann entwickelte Systemtheorie für eine empirische Analyse von Lärm und lärmerzeugenden Mechanismen geöffnet werden kann. Die dem Artikel zugrunde liegende analytische Strategie ist die Beobachtung der Operationen, die ein soziales System konstituieren. Eine entsprechende Analyse macht auf das Fehlen von Anschlusskommunikation und auf di...

The expression of genes involved in the pathogenesis of Staphylococcus aureus is known to be controlled by global regulatory loci, including agr, sarA, sae, arlRS, lytSR, and sarA-like genes. Here we described a novel transcriptional regulator called sarV of the SarA protein family. The transcription of sarV is low or undetectable under in vitro conditions but is significantly augmented in sarA...

Cereal seed development depends on the intimate interaction of filial and maternal tissues, ensuring nourishment of the new generation. The gene jekyll, which was identified in barley (Hordeum vulgare), is preferentially expressed in the nurse tissues. JEKYLL shares partial similarity with the scorpion Cn4 toxin and is toxic when ectopically expressed in Escherichia coli and tobacco (Nicotiana ...

Cell-free gene expression systems are emerging as an important platform for a diverse range of synthetic biology and biotechnology applications, including production of robust field-ready biosensors. Here, we combine programmed cellular autolysis with a freeze-thaw or freeze-dry cycle to create a practical, reproducible, and a labor- and cost-effective approach for rapid production of bacterial...

The fetus undergoes postmortem changes after death in utero due to autolysis. Several reports have suggested that the autolysis may provide a method for determining the intrauterine retention time (IURT).Among them, the Genest’s study has presented the loss of nuclear basophilia as a most reliable histologic indicator. According to the Genest’s criteria, 10 histologic features based on loss of ...

Growing cells of Clostridium saccharoperbutylacetonicum Nl-4 (ATCC 13564) were rapidly autolyzed in the presence of 0.3 to 0.5M sucrose. This autolysis was apparently induced by the treatment with sucrose, and termed “sucrose-induced autolysis.” The maximum effect was shown at 0.35M sucrose, exhib i t ing about 50% loss of initial turbidity of the cell suspension. The rate of lysis depended on ...