DNA quality is an important parameter for the detection and quantification of genetically modified organisms (GMO's) using the polymerase chain reaction (PCR). Food processing leads to degradation of DNA, which may impair GMO detection and quantification. This study evaluated the effect of various processing treatments such as heating, baking, microwaving, autoclaving and ultraviolet (UV) irrad...

STATEMENT OF PROBLEM Clinicians use the NiTi coil springs frequently for its appropriate mechanical properties. PURPOSE The aim of this study was to determine the effect of recycling and autoclave sterilization on the unloading forces of NiTi closed coil springs. MATERIALS AND METHOD Fourteen NiTi closed coil spring with the length of 9mm were selected. Each coil was stretched to a peak ext...

BACKGROUND The objectives of these laboratory experiments were: (1) to assess bromoxynil sorption, mineralization, bound residue formation and extractable residue persistence in a Dundee silt loam collected from 0-2 cm and 2-10 cm depths under continuous conventional tillage and no-tillage; (2) to assess the effects of autoclaving on bromoxynil mineralization and bound residue formation; (3) to...

Sterilized soils are commonly used for the study of xenobiotic sorption and as an abiotic control in biodegradation experiments. They are also used for the chemical study of nitrogen and carbon cycle processes and the elimination of the biological factors. In this research the effects of different soil sterilization methods such as autoclaving, fumigating and exposure to ultraviolet and microwa...

In the course of screening microbial fermentations for potential pharmaceutical activity, the extract from an unidentified fungus (Schering culture number SCF1082A) was found to inhibit Candida albicans. Various media and fermentation conditions were examined in an effort to maximize production of the antifungal agent. The conditions which proved to be the most productive are as follows. Stock ...

Alginate encapsu lation of an atoxigen ic strain of Aspergillu s ̄ avus was studied in order to optimize encapsu lation of fungal inocula with alginic acid . Steriliza tion by autoclav ing is known to depolymerize sodium alginate . Buffered solution s (pH 5 7± 8) reduced this effect . Autoclaving the alginate solutio n with a ® ller/nutrien t further inhibite d the depolymerization reaction . Au...

In spite of evident progress in the serology of Chagas disease, the requirement for new diagnostic antigens persists. We have evaluated different antigens obtained from Trypanosoma cruzi grown in medium rich in nutrients or under nutrient stress, autoclaved or sonicated and fractionated by differential centrifugation. The resulting antigens were evaluated for diagnosis of Chagas disease using E...

Biotechnology and pharmaceutical research have created a number of new and potentially life-saving drugs. Many of these drugs are formulated as injectable products. Some drug products do not survive autoclaving or other means of terminal sterilization. An aseptic filling process is typically used to sterilize such products, but it is less reliable than autoclaving, making detection of unsterile...

The causal infectious agents of TSEs (transmissible spongiform encephalopathies or prion diseases) are renowned for their resistance to complete inactivation. Survival of TSE infectivity after autoclaving potentially compromises many procedures where TSE infectivity may be present, including surgical instrument sterilization. In the present study, the heat inactivation properties of five differ...