We have examined the roles of the p85/ p110alpha and hVPS34 phosphatidylinositol (PI) 3'-kinases in cellular signaling using inhibitory isoform-specific antibodies. We raised anti-hVPS34 and anti-p110alpha antibodies that specifically inhibit recombinant hVPS34 and p110alpha, respectively, in vitro. We used the antibodies to study cellular processes that are sensitive to low-dose wortmannin. Th...

BACKGROUND Protein kinase C regulates various cellular processes including cell proliferation, cell adhesion, apoptosis, angiogenesis, invasion, and metastasis. Activation of different PKC isozymes results in distinct cellular responses. Novel PKCs are mainly involved in apoptotic process. Atypical PKC subfamily plays a critical role in cell proliferation and apoptosis, cell differentiation and...

We have examined the roles of the p85/ p110 a and hVPS34 phosphatidylinositol (PI) 3 9 -kinases in cellular signaling using inhibitory isoform-specific antibodies. We raised anti-hVPS34 and anti-p110 a antibodies that specifically inhibit recombinant hVPS34 and p110 a , respectively, in vitro. We used the antibodies to study cellular processes that are sensitive to lowdose wortmannin. The antib...

In this report, we review the pharmacological and non-pharmacological treatments of the different absence seizure types as recently recognized by the International League Against Epilepsy: typical absences, atypical absences, myoclonic absences, and eyelid myoclonia with absences. Overall, valproate and ethosuximide remain the principal anti-absence drugs. Typical absence seizures exhibit a spe...

the effect and arrangement of cytoskeleton (microtubules, microfilaments and neurofilaments) in neurulation of chick embryo examined by immunoperoxidase method from stage 6 to stage 12. the results were as follows: 1) microtubules aggregated in the perinuclear region from which they radiated to take longitudinal course beneath the membrane. 2) microfilaments were aggregated in the apical ends (...

Myasthenia Gravis (MG) is a neuromuscular junction disorder caused by pathogenic autoantibodies to some parts of the post-synaptic muscle endplates. About 85% of generalized MG patients have autoantibodies against post-synaptic acetyl-choline receptors (AChR). From the 10-15% of the remaining patients, 45-50% are positive for Muscle Specific Tyrosine Kinase-Antibody (MuSK-Ab). It is believed th...

Previous studies have shown that the viral oncogene product, v-Src, is localized to a perinuclear structure. Here, we demonstrate by immunofluorescence analysis that the perinuclear structure corresponds to a local concentration of vesicles. Overexpressed normal cellular Src, c-Src, and a temperature-sensitive mutant of v-Src also are associated with these perinuclear vesicles. This perinuclear...