High environmental hydrogen peroxide (H2O2) has been demonstrated to be toxic for fish. However, the response mechanism of fish chronic H2O2 exposure is not yet well understood. Therefore, this study aimed investigate alteration in ion transport gills and analyzed potential after exposure. The common carps were exposed 0, 0.25, 0.50, 1.00 mM 14 days. histopathological evaluation results indicat...

The kinetics and mechanism of action of rabbit skeletal muscle phosphofructokinase (ATP:D-fructose 6-phosphate 1 -phosphotransferase, EC 2.7.1.11) were investigated by several techniques. The initial velocity, product inhibition, isotope exchange patterns, and the result of a pulse labeling experiment are consistent with a mechanism in which ADP (or inosine diphosphate) dissociates from the enz...

The present communication reports the dose and exposure dependent effects of a dioxin on few ion dependent membrane bound ATPases of mice kidney. Effects of TCDD on the specific activity of few membrane bound ATPases viz., Total ATPase, Na K ATPase, Ca ATPase, Mg ATPase, Ca++, HCO3 ATPase, Mg, HCO3 ATPase were observed. Two basic hypotheses were tested viz., (a) the sublethal in vivo exposure o...

Disturbances of Na,K-ATPase activity are implicated in the pathophysiology of cerebral ischemia. Previous experiments have shown that EGb 761 protects NaK-ATPase activity against one hour of cerebral ischemia. In the brain however, the 3 isoenzymes responsible for Na,K-ATPase activity may be differentially affected by various times of ischemia. In the present study, we investigated the effect o...

Dopamine D1-like receptor activation causes phosphorylation and inhibition of Na,K-ATPase (Na-pump) activity in the proximal tubules, which is associated with an increase in sodium excretion. It has been shown that dopamine and SKF 38393, a D1-like receptor agonist, caused inhibition of Na,K-ATPase activity in the proximal tubules of adult (6 mo) but not of old (24 mo) Fischer 344 rats. The pre...

The mechanistic Target of Rapamycin complex 1 (mTORC1) senses intracellular amino acid levels through an intricate machinery, which includes the Rag GTPases, Ragulator and vacuolar ATPase (V-ATPase). The membrane-associated E3 ubiquitin ligase ZNRF2 is released into the cytosol upon its phosphorylation by Akt. In this study, we show that ZNRF2 interacts with mTOR on membranes, promoting the ami...