Forty-seven saturated synthetic diacyl PA, PC, PE, PG, and PS and five unsaturated diacyl phospholipids (PL) underwent normaland reversed-phase (RP) HPLC with isocratic isopropanol/hexane/water (5∶4∶1) and methanol/chloroform/acetonitrile/water (79.5∶9∶8∶3.5) mobile phases, respectively. For normal-phase HPLC, capacity factors (k′ i ) decrease with chain length (n) of the two identical PL FA re...

A stability-indicating, robust, fast, and user friendly reversed-phase high-performance liquid chromatographic (RP-HPLC) assay method has been developed and validated for the analysis of commercial raw material batches of methylparaben, ethylparaben, propylparaben, and butylparaben. These four parabens are widely used as preservatives in pharmaceutical and cosmetic products. Accurate assay valu...

Snake venoms comprise a highly complex mixture of proteins, and there is also high interspecific intraspecific variability in their composition, even the same region. Our aim was to compare composition Bothrocophias myersi, Crotalus durissus Bothrops asper, snakes from Andean region Colombia by Reverse-Phase High-Performance Liquid Chromatography (RP-HPLC). The were given research group under a...

The Arctic mutation interferes with processing of the amyloid precursor protein. The Arctic Alzheimer mutation favors intracellular Aȕ production by making APP less available to Į-secretase. Journal of Neurochemistry, in press.ȕ oligomers are inefficiently measured by enzyme-linked immunosorbent assay. and Johansson AS. Docosahexaenoic acid stimulates non-amyloidogenic APP processing resulting ...

High-performance liquid chromatography (HPLC) technique for cyamemazine tartrate (CYMT) was developed and validated according to the International Conference on Harmonization (ICH) Q2 (R1) guidelines. The method estimating CYMT from a bulk its pharmaceutical dosage form found be simple, precise, accurate, fast, stable reverse phase HPLC (RP-HPLC) approach. For chromatographic separation, Hypers...

A rapid and sensitive RP-HPLC method with UV detection (219nm) for routine analysis of Rifampicin in a pharmaceutical formulation was developed. Chromatography performed mobile phase containing methanol assay (99.8%) flow rate 0.9ml/min. Quantitation accomplished an internal standard method. The procedure validated linearity (correlation coefficient = 0.9957), accuracy limit (LOD) intraday prec...

A RP-HPLC assay method has been developed and validated for cefotaxime. An isocratic RP-HPLC was developed on a SS Wakosil II- C8 column (250 mm ˜4.6 mm i.d., 5 μm) utilizing a mobile phase of ammonium acetate buffer (pH 6.8) and acetonitrile (85:15 v/v) with UV detection at wavelength 252 nm at the flow rate 0 .8 ml/min. The proposed method was validated for sensitivity, selectivity, linearity...

Objective: The present aim is to develop simple, precise, and accurate reverse phase high performance liquid chromatographic method (RP-HPLC) for the simultaneous assay of artemether and lumefantrine in fixed combined dosage form. Methods: The chromatographic study was carried out on Hypersil C18 column (250×4.6 mm, 5 μ) with mobile phase containing a mixture of KH2PO4 buffer (pH-3.5) and aceto...