نتایج جستجو برای: aroa gene

تعداد نتایج: 1141529  

Journal: :Applied and environmental microbiology 2002
Siqing Liu James E Graham Lance Bigelow Philip D Morse Brian J Wilkinson

Listeria monocytogenes is a food-borne bacterial pathogen that is able to grow at refrigeration temperatures. To investigate microbial gene expression associated with cold acclimation, we used a differential cDNA cloning procedure known as selective capture of transcribed sequences (SCOTS) to identify bacterial RNAs that were expressed at elevated levels in bacteria grown at 10 degrees C compar...

Journal: :Proceedings of the National Academy of Sciences of the United States of America 1994
C M Khan B Villarreal-Ramos R J Pierce G Riveau R Demarco de Hormaeche H McNeill T Ali N Fairweather S Chatfield A Capron

A vector has been constructed to allow genetic fusions of guest antigens via a hinge domain to the C terminus of the highly immunogenic C fragment of tetanus toxin. A fusion has been constructed with the gene encoding the protective 28-kDa glutathione S-transferase (EC 2.5.1.18) from Schistosoma mansoni. The recombinant vector has been electroporated into the nonvirulent Salmonella typhimurium ...

Journal: :Biochimica et biophysica acta 2004
Rachel N vanden Hoven Joanne M Santini

Heterotrophic arsenite oxidation by Hydrogenophaga sp. str. NT-14 is coupled to the reduction of oxygen and appears to yield energy for growth. Purification and partial characterization of the arsenite oxidase revealed that it (1). contains two heterologous subunits, AroA (86 kDa) and AroB (16 kDa), (2). has a native molecular mass of 306 kDa suggesting an alpha(3)beta(3) configuration, and (3)...

Journal: :Vaccine 2009
Qi-long Wang Qin Pan Yunfeng Ma Kun Wang Ping Sun Shengwu Liu Xiao-Lian Zhang

There is a need to develop protective vaccines against tuberculosis (TB) that elicit full immune responses including mucosal immunity. Here, a live attenuated Salmonellatyphimurium aroA SL7207 vector TB vaccine, namely SL(E6-85B), harboring the Mycobacterium tuberculosis (M. tb) H37Rv ESAT6-Ag85B fusion gene was developed. The experimental data demonstrated that this SL(E6-85B) vaccine, or when...

Journal: :Journal of immunology 2014
Grefachew Workalemahu Hong Wang Kia-Joo Puan Mohanad H Nada Tomohisa Kuzuyama Bradley D Jones Chenggang Jin Craig T Morita

Human Vγ2Vδ2 T cells monitor isoprenoid metabolism by recognizing foreign (E)-4-hydroxy-3-methyl-but-2-enyl pyrophosphate (HMBPP), a metabolite in the 2-C-methyl-D-erythritol-4-phosphate pathway used by most eubacteria and apicomplexan parasites, and self isopentenyl pyrophosphate, a metabolite in the mevalonate pathway used by humans. Whereas microbial infections elicit prolonged expansion of ...

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