We investigated the effects of 1-beta-D-arabinofuranosylcytosine (ara-C) on the growth of murine leukemic L1210 cells, which were cultured with high (2.0 x 10(3) ng/ml), middle (100 ng/ml) and low doses (5.0 ng/ml) of ara-C. In the analysis by flow cytometry, high dose ara-C arrested the cell cycle in the G0/G1-phase. Middle and low doses ara-C induced a block in the S-phase, that was not compl...

Allergic reactions to peanuts and tree nuts are major causes of anaphylaxis in the United States. We compare different properties of natural and recombinant versions of Ara h 1, a major peanut allergen, through structural, immunologic, and bioinformatics analyses. Small angle x-ray scattering studies show that natural Ara h 1 forms higher molecular weight aggregates in solution. In contrast, th...

The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide c...

A reagentless electrochemical impedance biosensor for detection of peanut protein Ara h 1, one of the allergenic proteins found in peanuts, has been demonstrated using an Au substrate onto which an antibody film has been immobilized. Following initial stabilization of the self-assembled monolayer (SAM) through which the antibody is immobilized, the biosensor substrate exhibits stable impedance ...

The major peanut allergen Ara h 1 is an easily digestible protein under physiological conditions. The present study revealed that pepsin digestion products of Ara h 1 retained the sensitizing potential in a Brown Norway rat model, while this sensitizing capacity was lost by separating the digest into fractions by gel permeation chromatography. Protein chemical analysis showed that the peptide c...

There is little knowledge about the factors that determine the allergenicity of food proteins. One aspect that remains to be elucidated is the effect of the food matrix on immune responses to food proteins. To study the intrinsic immunogenicity of allergens and the influence of the food matrix, purified peanut allergens (Ara h 1, Ara h 2, Ara h 3, or Ara h 6) and a whole peanut extract (PE) wer...

Recent reports by Baccarani and Tura [2], Moloney and Rosenthal [9], and Housset et al. [6] prompted us to treat 12 acute myeloid leukemia (AML) and 4 myelodysplastic syndrome (MDS) patients with low dose cytosine arabinoside (Ara-C). In all patients, conventional chemotherapy was contraindicated because of previous inefficacy, advanced age, or heavy comorbidity. Ara-C was given in a dose of 10...