Dominant missense mutations in AQP5, encoding the water channel protein Aquaporin 5, underlie diffuse non-epidermolytic palmoplantar keratoderma (NEPPK), a rare skin condition characterized by thickening of epidermis palms and soles. Prior to this, AQP5 was only known be expressed sweat gland cells role this remains unclear. To study skin, we have two patient-derived keratinocyte cell lines exp...

Dysregulated sweat secretion can cause serious clinical problems. Experimentally regenerated human glands (hSWGs) should provide a valuable tool for dissection of hSWG biology and the pathophysiology sweating disturbance, and, ultimately drug discovery disorders; however, full establishment protocols bioengineering requires further endeavour. In this study, we attempted to establish methodology...

Cryoablation is an emerging type of treatment for cancer. The sensitization tumors using cryosensitizing agents prior to enhances ablation efficiency and may improve clinical outcomes. Water efflux, which regulated by aquaporin channels, contributes cancer cell damage achieved through cryoablation. An increase in (AQP) 3 cryoprotective, whereas its inhibition augments cryodamage. present study ...

Aquaporin (AQP)5, an exocrine-type water channel, was detected in the rat duodenum by Western blot analysis, and was localized by immunohistochemistry in the secretory granule membranes as well as in the apical and lateral aspects of the plasma membrane of Brunner's gland cells. Incubation of duodenal slices with vasoactive intestinal polypeptide (VIP) in vitro significantly increased the amoun...

PURPOSE To increase the database of genes expressed in human cornea and to gain insights into the molecular basis of keratoconus (KC). METHODS A cDNA library was constructed from KC corneas harvested at keratoplasty and used for expressed sequence tag (EST) analysis. Data were analyzed using grouping and identification of sequence tags (GRIST). Expression of selected clones was examined by RT...

The mouse granular convoluted tubules (GCTs), which are only located in the submandibular gland (SMG) are known to develop and maintain their structure in an androgen-dependent manner. We previously demonstrated that the GCTs are involuted by the epithelial deletion of core binding factor β (CBFβ), a transcription factor that physically interacts with any of the Runt-related transcription facto...

Fluid transport across epithelial and endothelial barriers occurs in the neonatal and adult lungs. Biophysical measurements in the intact lung and cell isolates have indicated that osmotic water permeability is exceptionally high across alveolar epithelia and endothelia and moderately high across airway epithelia. This review is focused on the role of membrane water-transporting proteins, the a...

Aquaporin-8 (AQP8) is a water-transporting protein expressed in organs of the mammalian gastrointestinal tract (salivary gland, liver, pancreas, small intestine, and colon) and in the testes, heart, kidney, and airways. We studied the phenotype of AQP8-null mice, and mice lacking AQP8, together with AQP1 or AQP5. AQP8-knockout mice lacked detectable AQP8 transcript and protein, and had reduced ...

Using primers against highly conserved regions of mammalian and bird aquaporins in RT-PCR experiments, we amplified products derived from duck (Anas platyrhynchos) nasal gland RNA that were identified as homologues of mammalian and chicken aquaporin 1 and aquaporin 5 cDNAs by sequencing. Using digoxigenin-labelled probes derived from these PCR products in northern blot analyses of mRNA isolated...