Cherry plum (Prunus divaricata Ledeb.) is a diploid fruit tree that belongs to the Rosaceae family. Anticarcinogenic activity of Prunus Ledeb. methanol extract was examined in lung cancer cell lines A549 and H1299 as well healthy line Beas-2b this study. The changes expression levels most frequently mutated oncogenes cancer, KRAS, EGFR, BRAF, PIK3CA were investigated well. In vitro cytotoxicity...

Abstract The Apoptosis is an intensely studied mechanism for cancer therapy. Various genes, drugs, and molecules have been combined to potentiate the apoptotic effect against tumor cells. Magnetic nanocomplexes with biopolymers proposed as nanocarriers delivery of active improve apoptosis induction. To these purpose were formulated magnetic nanoparticles curcumin coated chitosan (MNPs-CHI-TPP-C...

The two subtypes of melanomas, uveal (UM) and cutaneous (CM), originate from melanocyte transformation, but differ in their genetic etiology signaling pathways. MCL1 BCL2, the anti-apoptotic members BCL2 family, induce anti-cancer treatment resistance. Here, we evaluate basal levels role inducing response to inhibitor (MCL1i) CM UM. We used vitro assays (viability, immunoblot shRNA), bioinforma...

Aim: To elucidate the anti-apoptotic properties of nuclear factor kappa light-chain-enhancer of activated B cells (NF-κB) and feedback regulation of NF-κB by nuclear factor of kappa light-chain-enhancer of activated B-cells inhibitor alpha (IκBα). Methods: We developed an in vitro model of Sjogren’s syndrome by transfecting human salivary gland (HSG) and acinar cells (NS-SV-AC) with a plasmid-e...

This study explored the changes in bone marrow CD235a+ nucleated red blood cell apoptosis and signal pathways patients with chronic mountain sickness. We collected data from 21 sickness 20 non-chronic patients. Bone mononuclear cells, flow cytometry is used to detect apoptotic index of cells determine mitochondrial membrane potential; reverse transcription quantitative polymerase chain reaction...

Recent studies suggest that controlled apoptotic response provides an essential mechanism, enabling corals to respond to global warming and ocean acidification. However, the molecules involved and their functions are still unclear. To better characterize the apoptotic response in basal metazoans, we studied the expression profiles of selected genes that encode for putative pro- and anti-apoptot...

Palmitic acid (PAM), one of the most common saturated fatty acid (SFA) in animals and plants, has been shown to induce apoptosis in exocrine pancreatic AR42J cells. In this study, we investigated cellular mechanisms underlying protective effects of oleic acid (OLA) against the lipotoxic actions of PAM in AR42J cells. Exposure of cells to long-chain SFA induced apoptotic cell death determined by...