To test directly whether fibrin(ogen) is a key binding site for apolipoprotein(a) [apo(a)] in vessel walls, apo(a) transgenic mice and fibrinogen knockout mice were crossed to generate fibrin(ogen)-deficient apo(a) transgenic mice and control mice. In the vessel wall of apo(a) transgenic mice, fibrin(ogen) deposition was found to be essentially colocalized with focal apo(a) deposition and fatty...

Nonenzymatic glycation of low density lipoprotein (LDL) is a reaction of glucose and other reducing sugars with apolipoprotein B100 (apo-B100) lysine residues. In diabetes, this reaction is greatly accelerated and is important in the pathogenesis of diabetic complications. The objective of this study was to investigate in vitro effects of ?-tocopherol, ascorbic acid and lycopene on LDL glycatio...

Apolipoprotein E (Apo E) is a polymorphic secretory glycoprotein expressed by a wide variety of tissues in mammals, particularly the liver and the brain (Mahley, 1988). Apo E recently attracted considerable attention when it was found that one of its alleles, apo E4, was associated with an increased risk for Alzheimer's disease (Strittmatter et al. 1993 a). This protein has been well studied wi...

OBJECTIVES In this study, we examined whether a reconstituted high-density lipoprotein (HDL) utilizing recombinant apolipoprotein A-I(Milano) (apo A-I(M))/phospholipid complex (PC) could restore normal endothelial function in hypercholesterolemic apolipoprotein (apo) E-null mice. BACKGROUND We have previously shown antiatherosclerotic and vasculoprotective effects of recombinant apo A-I(M). ...

The plasma high-density lipoproteins (HDL, rho 1.085-1.21 g/ml) of rainbow trout (Salmo gairdneri) have been shown to contain large amounts of apolipoprotein (apo) AI (Mr 28,000) and several other apolipoproteins of Mr less than 14,000 (apo C-like) and of Mr 37,000-38,000, 44,000-45,000 and 53,000-54,000. Comparison of apo AI from trout and human HDL shows them to be similar in Mr and to have s...

Synthesis and secretion of apolipoproteins in pig small intestine was studied by pulse-chase labeling of jejunal segments, kept in organ culture. Apo A-1 and apo B-48 were the two major proteins released, constituting 25 and 10%, respectively, of the total amount of labeled protein in the mucosal-side medium where they appeared with a t1/2 of 50-60 min. Using tissue from fasting animals, > 85% ...

Coronary artery disease (CAD) is a major cause of morbidity and mortality in modern society. Known risk factors for coronary artery disease include dyslipidemia, hypertension, obesity, dysglycemia, insulin resistance, inflammation, arterial intimal thickening, development of vulnerable plaque and thrombosis. This study compared lipid parameters in CAD patients with healthy individuals to see th...

A review of radioimmunoassays for measuring human apolipoprotein B (apo B), the A apolipoproteins of high density lipoprotein (apo A-I and apo A-II) and apolipoprotein C-II (apo C-II) in human plasma and in isolated lipoproteins is presented. The sensitivity, specificity and validity of each of these assays is discussed. In normolipidemic subjects the reported serum apo B concentrations ranged ...

We have previously shown that lipoprotein(a) (Lp(a)) assembly involves an initial noncovalent interaction between sequences within apolipoprotein(a) (apo(a)) kringle IV types 5-8 and the amino terminus of apolipoprotein B-100 (sequences between amino acids 680 and 781 in apoB-100), followed by formation of a disulfide bond. In the present study, citraconylation of lysine residues in apoB-100 ab...

background: to evaluate the association of apolipoprotein b (apob), apolipoprotein a (apoa), and apob/apoa ratio with the body fat indicators in patients with stable angina pectoris (sa). materials and methods: one hundred and twenty two participants aged 40-60 years old, with a mean age of 52.1 ± 7.2 years and sa, were recruited for the present study. body weight, height, and waist circumferen...