That some strains of the coliform group of bacteria exert inhibitory effects on other microorganisms, when grown in association in liquid media, was reported first by Nissle in 1916. Because of the multiplicity of factors involved when organisms are growing in heterogeneous mixtures in liquid media, the nature of the inhibiting agent is difficult to ascertain. Gratia of Belgium (1925) observed ...

Two paper strips, each containing different antimicrobial agents, were placed on plates on Mueller-Hinton agar to permit antibiotic to enter the agar. A filter membrane was placed on this plate, and the microorganisms were planted on the membrane. After 6 h of incubation at 37 C, the membrane was transferred to antibiotic-free Mueller-Hinton agar containing triphenyltetrazolium hydrochloride an...

Cefuroxime, a new antibiotic derived from cephalosporin-C which is very stable in the presence of beta-lactamases produced by Gram-negative organisms, was used to treat 856 male patients and 340 female patients with uncomplicated gonorrhoea. In men the drug was used in three different doses, 1.5 g, 1 g, and 750 mg plus 1 g probenecid; in women 1 g and 750 mg cefuroxime plus 1 g probenecid was u...

background and objectives: the prevalence of vibrio parahaemolyticus has been reported from ponnani earlier, however incidence of multidrug resistant strains have been encountered recently in clinical laboratories. the source for such strains and their presence in this major fish landing centre has been investigated. materials and methods: antibiotic sensitivity tests on isolates of v. parahaem...

Two mutants of the tylosin-producing Streptomyces fradiae defective in the biosynthesis of the macrolide antibiotic tylosin were isolated from colonies derived from regenerated protoplasts. Both strains were unable to carry out any of at least seven tylosin biosynthetic steps and were sensitive to tylosin. One strain, JS82, was also more sensitive to chloramphenicol (Cm), mitomycin C (Mc), hygr...

Dental plaque known to harbour Bacteroides melaninogenicus was removed from the gingival half of selected buccal tooth surfaces. The plaque was dispersed, diluted and cultured on medium containing 0 per cent, 0*05,0* 1 and 0.5 per cent Kanamycin. When the medium contained 0.05 per cent Kanamycin, only B. melaninogenicus were found and the number recovered was about 20 per cent of the B. melanin...

This study was conducted to determine whether there was any genetic heterogeneity among Helicobacter pylori strains isolated from the antrum and corpus of the same individual in a Malaysian population and to determine the presence of heterogeneous susceptibility of the isolates by comparing PCR-RAPD and antibiotic profiles. Forty-four H. pylori isolates cultured from the antrum and corpus of 22...

Clostridium difficile (C. difficile) infection (CDI) rates have exhibited a steady rise worldwide over the last two decades and the infection poses a global threat due to the emergence of antibiotic resistant strains. Interstrain antagonistic interactions across the host microbiome form an important strategy for controlling the emergence of CDI. The current diagnosis method for CDI, based on im...