To facilitate physical genome mapping, we have developed a new cytogenetic photomap for Anopheles stephensi (Liston) (Diptera: Culicidae), an important malaria vector in Asia. The high-resolution images of the ovarian polytene chromosomes have been straightened and divided by numbered divisions and lettered subdivisions. The exact chromosomal locations of eight DNA probes have been determined b...

Using the Anopheles gambiae Giles genome as a template, we designed, screened and identified 14 novel Exon-Primed Intron-Crossing (EPIC) PCR primer pairs for Anopheles pseudopunctipennis Theobald 1901, a major vector of human Plasmodium sp. in South America. These primers were designed to target the conserved regions flanking consecutive exons of different genes and enabled the amplification of...

Surveys were conducted in malaria-endemic villages in the southern province of Attapeu, Lao PDR during various seasons over a 3-year period. All-night mosquito landing collections, blood surveys and a case-control study were conducted. Plasmodium falciparum was the predominant species, and slide positivity rates were higher during the transition/dry season compared with the wet season. Anophele...

OBJECTIVE To investigate the larvicidal efficacy of different extracts of Ficus benghalensis L. against Culex quinquefasciatus Say, Aedes aegypti L. and Anopheles stephensi L. (Diptera: Culicidae). MATERIALS AND METHODS Larvicidal efficacy of the crude leaf extracts of Ficus benghalensis with three different solvents like methanol, benzene and acetone was tested against the early second, thir...

Environmental concerns have resulted in the search for environmentally friendly natural oils for use as mosquito larvacide. Methylated coconut oil has been found to be toxic to mosquito larvae. However, the use of methylated coconut oil is limited by resource constraints in rural communities in Papua New Guinea and other Pacific Island countries where coconut oil is produced by traditional meth...

This study examined the larval breeding habitat of a major South American malaria vector, Anopheles darlingi, in areas with varying degrees of ecologic alteration in the Peruvian Amazon. Water bodies were repeatedly sampled across 112 km of transects along the Iquitos-Nauta road in ecologically varied areas. Field data and satellite imagery were used to determine the landscape composition surro...

Malaria is the most important tropical disease that causes enormous economic loss to mankind. Malaria is an acute disease caused by parasites in human blood, namely Plasmodium. Four human malaria parasites were found among which Plasmodium falciparum is the most common species and may lead to mortality. The disease is transmitted by anopheles mosquitoes. Globally, there are some 60 anopheles mo...

The Mariana Islands lie well within the vast "malaria-free" region of the central and south Pacific Ocean. However, Anopheles indefinitus was discovered on Guam in 1948, and an additional four species of Anopheles were collected on the island during 1970-75. Early malaria cases on Guam were diagnosed as relapsing infections with the disease having been contracted in other areas. Small outbreaks...

Continuous monitoring of vector species composition, abundance, dynamics, feeding pattern, and host finding strategy is the base to determine when, what, and how control should be implemented. Thus, this study was conducted to assess entomological parameters of anopheline mosquitoes in nine villages in Seka district, southwestern Ethiopia, from June to December 2012. Mosquito collection was car...