Cytosolic glutathione peroxidase (GPX-1) is an important antioxidant enzyme that scavange hydrogen peroxide in mammalian cells. The level of GPX-1 activity in Japanese monkey ( Macaca fuscata ) tissues was determined and it was found to be high in the liver, kidney, and adrenal gland followed by the small intestine. We also cloned the GPX-1 cDNA that included the whole protein-coding region. Th...

Background: The pathogenesis of ethanol in the liver and kidney is associated with an increase in free radicals and oxidative stress that leads to structural and functional abnormalities in these vital organs. Methods: Forty-eight male wistar rats were placed in eight groups and were treated as follows: control group (normal saline), group of ethanol (4 g/ kg), three doses of Ardeh and honey co...

We have evaluated the effects of an oral treatment of mice with fraxetin (25 mg/kg for 30 days) on the glutathione system (GSH, GSSG, and GSSG/GSH ratio as stress index), glutathione reductase (GR) and glutathione peroxidase (GPx) in liver supernatants from male C57BL/6J mice (18-month old). A significant antioxidant effect in vivo was found under this treatment by a decrease in the GSSG/GSH ra...

Background: The aim of this study was to evaluate the possible protective effect of sodium selenite on serum, liver, and kidney antioxidant enzymes activities in alloxan-induced type 1 diabetic rats. Methods: Forty Sprague-Dawley male rats were randomly divided into four groups; Group one as control, Group two as sham-treated with sodium selenite by 1 mg/kg intraperitoneal (i.p.) injections ...

Methylmercury (MeHg) is an environmental neurotoxicant whose molecular mechanisms underlying toxicity remain elusive. Here, we investigated molecular events involved in MeHg-induced neurotoxicity in cultured cerebellar granule cells (CGCs) as well as potential protective strategies for such toxicity. Glutathione peroxidase, isozyme 1 (GPx-1) activity was significantly (p = 0.0017) decreased at ...

Oxidative stress plays an important role in obesity-related metabolic diseases. Glutathione peroxidase (GPX) is an antioxidant enzyme downregulated in adipose tissue of obese mice. However, the role of GPX in adipocytes remains elusive. The objective of this study was to clarify the pathophysiological changes in GPX activity and glutathione metabolism and their roles in the pathogenesis of insu...

Introduction Oxidative stress refers to an imbalance between oxidant and antioxidant activity accumulation of reactive oxygen species, which can have detrimental effects on animal health. Annual fluctuations in oxidative status occur, increasing disease susceptibility during certain time periods. However, a full understanding factors related Asian elephants how mitigate the negative consequence...

Cytosolic glutathione peroxidase (GPX-1) is an important antioxidant enzyme that scavange hydrogen peroxide in mammalian cells. The level of GPX-1 activity in Japanese monkey (Macaca fuscata) tissues was determined and it was found to be high in the liver, kidney, and adrenal gland followed by the small intestine. We also cloned the GPX-1 cDNA that included the whole protein-coding region. The ...

this work analyzes the effects of satureja khuzestanica essential oil (skeo) on the thyroid and antioxidant system, assessed by measuring levels of tri-iodothyronine (t3), thyroxine (t4), thyroid-stimulating hormone (tsh), malondialdehyde (mda), reduced glutathione (gsh), and glutathione peroxidase (gpx) activity. forty adult male sprague dawley rats (225 ± 25 g) were divided into five equal gr...

BACKGROUND Oxidative stress plays a role in the pathophysiology of several diseases and has been documented as a contributor to disease in both the human and veterinary literature. One at-risk cell is the erythrocyte, however, the role of oxidative stress in anemia in dogs has not been widely investigated. HYPOTHESIS/OBJECTIVE Anemic dogs will have an alteration in the activity of glutathione...