When embryonated and de-embryonated cotyledons of cowpea (Vigna unguiculata L.) were kept for germination, only embryonated cotyledons (EC) developed into seedlings. alpha-Amylase activity appeared late in de-embryonated cotyledons (DEC), but increased and matched with that of EC on 4th day, and thereafter started declining. A higher content of reducing sugars may be one of the factors in down ...

BACKGROUND The routine measurement of pleural fluid amylase is frequently recommended, but the cost-effectiveness of this procedure is unknown. METHODS To assess the utility of routine measurement of pleural fluid amylase in evaluating pleural effusions, we measured amylase, glucose, lactate dehydrogenase, and protein levels and blood cell counts in 379 patients undergoing thoracentesis durin...

Human serous-type ovarian tumors contain an acidic isoenzyme of amylase. Previous attempts at purification of tumor amylases have yielded preparations contaminated with other proteins. The purification scheme presented here incorporates an affinity-chromatography procedure, with use of cycloheptaamylose linked to epoxy-activated Sepharose, that is specific for alpha-amylase (EC 3.2.1.1). Purifi...

The intra- and extracellular concentrations of alpha-amylase in Aspergillus oryzae have been measured during batch culture of a wild-type strain and two recombinant strains. The mean intracellular level for the two recombinant strains was about four to five times the level of the wild-type strain. The recombinant strains also had a higher alpha-amylase productivity, whereas the residence time o...

Amylases are an important family of enzymes involved in insect carbohydrate metabolism that are required for the survival of insect larvae. For this reason, enzymes from starch-dependent insects are targets for insecticidal control. PF2 (Olneya tesota) is a lectin that is toxic to Zabrotes subfasciatus (Coleoptera: Bruchidae) larvae. In this study, we evaluated recognition of the PF2 lectin to ...

Alpha-amylase E.C.3.2.1.1. (1,4 glucan, 4 glucanohydrolase) can be obtained from salivary glands, pancreas and microorganisms such as Pseudomonas and Asperigllus, as well as muscles and ovarian tubes.1.2 Alpha-amylase from Bacillus Subtilis #1024(A TCC 465)* was purified with a highest degree of purity in our laboratory (31.59 U/mg). The extracellular alpha-amylase was subjected to differe...

Background: Breast cancer is one of the most diagnosed cancers in females, frequently with fatal outcome, so that new strategies for modulating cell proliferation in the mammary tissue are urgently needed. There is some, as yet inconclusive evidence that a-amylase may constitute a novel candidate for affecting cellular growth. Methods: The present investigation aimed to examine if salivary a-am...

introduction: α-amylase is a major form of amylase found in humans and other mammals. it is the special key enzyme involved in carbohydrates breakdown. inhibition of this enzyme could be used in treatment of diabetes. in this study, the effect of ten iranian macrolichens on alpha amylase were tested. methods: different concentrations of the extracts (25, 50 and 75 mg/ml) were incubated with enz...

Macroamylasemia is characterized by an elevated serum amylase activity. It results from the circulation of a macromolecular complex consisting of amylase molecule and a serum component, often an immunoglobulin. The present study presents marked elevation of serum amylase level in a 43-year-old male, who had been admitted to our hospital for slight abdomen ache for 4 months. After treatment foll...

OBJECTIVES One strategy for the treatment of diabetes is inhibition of pancreatic α- amylase. Plants contains different chemical constituents with potential for inhibition of α-amylase and hence maybe used as therapeutic. MATERIALS AND METHODS Urtica dioica and Juglans regia Linn were tested for α-amylase inhibition. Different concentrations of leaf aqueous extracts were incubated with enzyme...