Active efflux of antimicrobial agents is a primary mechanism by which bacterial pathogens can become multidrug resistant. The combined use of efflux pump inhibitors (EPIs) with pump substrates is under exploration to overcome efflux-mediated multidrug resistance. Phenylalanine-arginine β-naphthylamide (PAβN) is a well-studied EPI that is routinely combined with fluoroquinolone antibiotics, but ...

results the present study indicates that carbapenems and new cephalosporin antibiotics were practically ineffective against the extensive drug resistance (xdr) strains. colistin was observed to be more effective, although in seven cases resistance to colistin observed. ampc β-lactamase and mbl could be an important contributory factor for imipenem resistance among the isolates in our hospital. ...

Extended-spectrum-β-lactamase (ESBL)/AmpC producing Enterobacteriaceae have been reported worldwide amongst isolates obtained from humans, food-producing animals, companion animals, and environmental sources. However, data on prevalence of fecal carriage of ESBL/AmpC producing Enterobacteriaceae in healthy companion animals is limited. This pilot study describes the prevalence of ESBL/AmpC enco...

Food contamination with extended-spectrum beta-lactamase (ESBL)-producing bacteria is recognized as one of the public health concerns worldwide. Although fish and fishery products at retail in Japan have relied on imported materials, no apparent evidences exist prevalence property genetic transfer ESBL-producing those food specimens. This study aimed to investigate plasmid-mediated antibiotic-r...

Cloacal swabs from carcasses of Dutch wild birds obtained in 2010 and 2011 were selectively cultured on media with cefotaxime to screen for the presence of extended-spectrum β-lactamase (ESBL)/AmpC-producing Escherichia coli. Subsequently, all cefotaxime-resistant E. coli isolates were tested by broth microdilution and microarray. The presence of ESBL/AmpC and coexisting plasmid-mediated quinol...

Dear Sir, Enterobacteriaceae, other than Escherichia spp. and Klebsiella spp., can cause serious nosocomial infections and appear to have become increasingly drug resistant. Citrobacter freundii, belonging to the Enterobacteriaceae family, is a prominent opportunistic pathogen responsible for serious infections in immunocompromised individuals. C. freundii is characterized by the presence of ch...

Escherichia coli isolate MEV, responsible for a bloodstream infection, was resistant to penicillins, cephalosporins, and ertapenem. Molecular and biochemical characterization revealed the production of a novel, chromosome-borne, extended-spectrum AmpC (ESAC) β-lactamase with a Ser-282 duplication and increased carbapenemase activity. This study demonstrates for the first time that chromosome-bo...

A population analysis of 103 multidrug-resistant Pseudomonas aeruginosa isolates from Croatian hospitals was performed. Twelve sequence types (STs) were identified, with a predominance of international clones ST235 (serotype O11 [41%]), ST111 (serotype O12 [15%]), and ST132 (serotype O6 [11%]). Overexpression of the natural AmpC cephalosporinase was common (42%), but only a few ST235 or ST111 i...

not analyze changes in the outer membrane proteins responsible for alteration of permeability. Continual monitoring of drug resistance patterns is imperative. Antimicrobial drug susceptibility testing should be conducted for clinical isolates, and empirical antimicrobial drug therapy should be changed accordingly. AmpC β-lactamase genes will eventually be transferred to typhoidal salmonellae, w...

conclusions the potential to form biofilm correlated with antibiotic resistance and ampc production in non-burn burn isolates of a. baumannii. on the other hand, the burn strains produced significantly higher amounts of esbl yet biofilm production was unrelated to antibiotic resistance or esbl-production. materials and methods sixty isolates of a. baumannii were employed of which, 30 were burn ...