Abacavir sulphate was subjected to forced degradation under the conditions of hydrolysis (acid, alkali and neutral), oxidation, photolysis and thermal stress as prescribed by ICH. Eight degradation products were formed and their separation was accomplished on Waters XTerra C₁₈ (250 mm x 4.6 mm, 5 μm) column using 20 mM ammonium acetate:acetonitrile as a mobile phase in gradient elution mode by ...

Two simple, accurate and precise methods are described for the determination of propranolol hydrochloride with cerium(IV) sulphate. The titrimetric method is based on the oxidation of the drug by a known excess amount of cerium(IV) sulphate and back titration of the unconsumed oxidant with ammonium ferrous sulphate. In the spectrophotometric method, the unreacted cerium(IV) sulphate is treated ...

DSDP/ODP porewater profiles in organic carbon-bearing (< 5% org. C) sediments commonly show decreases in Ca concentrations and increases in alkalinity over depths where sulphate is being removed by microbial reduction. These Ca depletion profiles represent the combined effect of diffusion, advection and reaction (addition by ion exchange and removal by precipitation mainly as CaCO3 and/or dolom...

One of the challenges to be confronted during landfill operations is to handle the landfill leachate with high ammonium content. Current biological ammonium removal processes either are complicated and hard to manage, or require major investments. Low cost and low maintenance onsite systems for the treatment of landfill leachate with high ammonium content are in urgent need, especially for land...

Chamber studies of glyoxal uptake onto ammonium sulphate aerosol were performed under dark and irradiated conditions to gain further insight into processes controlling glyoxal uptake onto ambient aerosol. Organic fragments from glyoxal dimers and trimers were observed within the aerosol under dark and irradiated conditions. Glyoxal monomers and oligomers were the dominant organic compounds form...

alpha 1-Proteinase inhibitor was purified from porcine blood by ammonium sulphate and Cibachron Blue-Sepharose fractionation, ion exchange chromatography on DEAE-Cellulose, gel filtration on Sephadex G-25, and zinc chelating chromatography. Thus, an inhibitor preparation with a specific activity of 1.62 IU/mg protein (enzyme: trypsin; substrate: BzArgNan) was obtained. In sodium dodecyl sulphat...

hbs vaccines were prepared by three different methods: 1) heat inactivation (krugman, et.al 1971)2) ammonium sulphate precipitation followed by column chromatography (sephadex g-200) 3) peg-6000 (polyethylene glycol) precipitation followed by column chromatography using sephadex g-200. their efficacy was studied in guinea pigs, and the results compared with commercial japanese vaccine (green cr...

Carboxymethyl cellulase (CMCase) provides a key opportunity for achieving tremendous benefits of utilizing rice straw as cellulosic biomass. Out of total 80 microbial isolates from different ecological niches one bacterial strain, identified as Bacillus sp. 313SI, was selected for CMCase production under stationary as well as shaking conditions of growth. During two-stage pretreatment, rice str...

A hygromycin B phosphotransferase activity from Streptomyces hygroscopicus has been highly purified by ammonium sulphate fractionation followed by affinity column chromatography through Sepharose-6B-hygromycin-B. The combined active fractions showed a single protein band (41 kDa) when subjected to polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. When gel electropho...