Diabetes mellitus is a multifactorial disease. The nature has a plentiful sources as a remeady for this slow-killing condition. The aim of the present to assess the medicinal plants for anti-diabetic activity by using -amylase and -glucosidase inhibition assay. Anti-diabeticactivities of medicinal plants weredetermined by -amylase and -glucosidase inhibition assay. Results revealed that methono...

The use of α-glucosidase inhibitors is considered to be an effective strategy in the treatment of diabetes. Using a bioassay-guided fractionation technique, five Bacillus stearothermophilus α-glucosidase inhibitors were isolated from the flowers of Musa spp. (Baxijiao). Using NMR spectroscopy analysis they were identified as vanillic acid (1), ferulic acid (2), β-sitosterol (3), daucosterol (4)...

Enzymatic cleavage of the glycosidic bond yields products in which the anomeric configuration is either retained or inverted. Each mechanism reflects the dispositions of the enzyme functional groups; a facet of which is essentially conserved in 113 glycoside hydrolase (GH) families. We show that family GH97 has diverged significantly, as it contains both inverting and retaining alpha-glycosidas...

This study was performed to establish anti-diabetic and anti-oxidant properties, carry out phytochemical analysis of selected local plants which are traditionally used as medicinal plants. Local involved in this were Lawsonia inermis, Punica granatum, Dryobalanops aromatica, Ziziphus mauritiana, Ocimum basilicum. Solvent extraction using maceration method with solvents increasing polarity. Alph...

A soil microorganism producing αand β-glucosidase inhibitors was identified as Bacillus lentimorbus, based on the fatty acid and morphological analyses, along with biochemical and physiological tests. The α-glucosidase inhibitor was highly produced by this strain in a culture medium containing 0.25% of sodium glutamate and 0.5% of glucose, pH 8.0 at 30C for 2 days. The α-glucosidase inhibitor f...

Four cultures of a Candida sp. that lacked alpha-glucosidase activity were isolated from clinical specimens. Physiological, morphological, and serological characterizations of the yeasts and deoxyribonucleic acid reassociation studies supported their classification as a variant of C. tropicalis.