Two mutants of the Escherichia coli F1 ATPase, betaY331W:E381C/epsilonS108C and alphaS411C/betaY331W/epsilonS108C, have been used to relate nucleotide binding in catalytic sites with different interactions of the stalk-forming subunits gamma and epsilon at the alpha3beta3 subunit domain. Essentially full yield cross-linking between beta + gamma and beta + epsilon, or between alpha + gamma and a...

The 3'-5' circular trinucleotide cr(GpGpGp) was studied by means of 1D and 2D high resolution NMR techniques and molecular mechanics calculations. Analysis of the J-couplings, obtained from the 1H and 13C-NMR spectra, allowed the determination of the conformation of the sugar rings and of the 'circular' phosphate backbone. In the course of the investigations it was found that the Karplus-equati...

The experimental results of isothermal pressure dielectric permittivity epsilon studies in a critical mixture characterized by a negative shift of critical temperature induced by pressure (dT(C)/dP<0) are presented. The critical effect is portrayed by the same relation as in previous epsilon(T) and epsilon(P) studies, with the critical exponent alpha=0.12+/-0.03. The advantage of pressure studi...

From a smooth, strictly convex function phi: Rn --> R, a parametric family of divergence function Dphi(alpha) may be introduced: [ equation: see text] for x, y epsilon int dom (Phi) subset Rn, and for alpha in R, with Dphi(+/-1) defined through taking the limit of alpha. Each member is shown to induce an alpha-independent Riemannian metric, as well as a pair of dual alpha-connections, which are...

To characterize the function of the CD3 eta subunit of the T cell receptor (TCR), we have used cDNAs encoding CD3 zeta, CD3 eta, or both to reconstitute a variant of a cytochrome c-specific, I-Ek-restricted murine T cell hybridoma, termed MA5.8, which lacks CD3 zeta and CD3 eta proteins. We provide direct evidence that assembly and surface expression of TCRs can be mediated by either of these s...

Measurement of the dielectric permittivity of colloidal suspensions in the kilohertz frequency range (the so-called low-frequency dielectric dispersion) is a promising tool for the electrokinetic characterization of colloids. However, this technique is less used than would be desirable because of the difficulties associated with the measurements, the most important of which is the electrode pol...

We study homogenization of a boundary obstacle problem on $ C^{1,\alpha} domain $D$ for some elliptic equations with uniformly coefficient matrices $\gamma$. For any \epsilon\in\mathbb{R}_+$, $\partial D=\Gamma \cup \Sigma$, $\Gamma \cap \Sigma=\emptyset and S_{\epsilon}\subset \Sigma suitable assumptions,\ we prove that as $\epsilon$ tends to zero, the energy minimizer u^{\epsilon} \int_{D} |\...

Cross-linking reagents have been used to link covalently adjacent subunits of solubilized spinach chloroplast coupling factor 1, which is a latent ATPase. 1,5-Difluoro-2,4-dinitrobenzene, dimethyl-3,3'-dithiobispropionimidate, and dimethylsuberimidate are able to form bridges of 3 to 11 A between amino groups, and hydrogen peroxide and the o-phenanthroline-cupric ion complex catalyze the oxidat...

The determination of long segments of DNA sequences encompassing the beta- and alpha-globin gene clusters has provided an unprecedented data base for analysis of genome evolution and regulation of gene clusters. A newly developed computer tool kit generates local alignments between such long sequences in a space-efficient manner, helps the user analyze the alignments effectively, and finds cons...