certainly possible to improve the results of medical treatment in our present state of knowledge. First the diagnosis of ulcer should be established as early as possible after symptoms begin and thorough treatment with bed rest should be given at the onset; how rarely does one see a patient in a hospital bed with an ulcer history of less than a year? It is in this respect that our professional ...

Intracellular microelectrode techniques and extracellular pH measurements were used to study the dependence of apical Na+/H+ exchange on mucosal and intracellular pH and on mucosal solution Na+ concentration ([Na+]o). When mucosal solution pH (pHo) was decreased in gallbladders bathed in Na(+)-containing solutions, aNai fell. The effect of pHo is consistent with titration of a single site with ...

We used microelectrodes to monitor the recovery (i.e., decrease) of intracellular pH (pHi) after using internal dialysis to load squid giant axons with alkali to pHi values of 7.7, 8.0, or 8.3. The dialysis fluid (DF) contained 400 mM K+ but was free of Na+ and Cl-. The artificial seawater (ASW) lacked Na+, K+, and Cl-, thereby eliminating effects of known acid-base transporters on pHi. Under t...

Measurements of cytosolic pH (pHi) 36Cl fluxes and free cytosolic Ca2+ concentration ([Ca2+]i) were performed in the clonal osteosarcoma cell line UMR-106 to characterize the kinetic properties of Cl-/HCO3- (OH-) exchange and its regulation by pHi and [Ca2+]i. Suspending cells in Cl(-)-free medium resulted in rapid cytosolic alkalinization from pHi 7.05 to approximately 7.42. Subsequently, the ...

Tumor lysis syndrome can be observed when rapidly dividing, large-volume tumors such as high-grade lymphomas and acute leukemias are treated with cytotoxic agents.’ The syndrome is heralded by hyperuricemia, hyperkalemia, hyprphosphatemia, hypocalcemia, and renal failure. Two cases have been previously described of tumor lysis in prolymphocytic leukemia (PLL). Both cases were unexpected given t...

RITCHIE, A. E. (National Animal Disease Laboratory, Ames, Iowa), AND HERMAN C. ELLINGHAUSEN. Electron microscopy of leptospires. I. Anatomical features of Leptospira pomona. J. Bacteriol. 89:223-233. 1965.-Anatomical features of Leptospira pomona are described. Most cells in the logarithmic phase of growth, when examined in whole cell "negative-stained" preparations, display a homogeneous finel...

A serologically active substance was extracted from cells of Hemophilus aegyptius with dilute NAOH. This substance directly hemagglutinated human erythrocytes. The activity of this material was demonstrated at a near neutral pH and was precipitated at acid pH. Preliminary biochemical determination showed this serologically active material to be predominately polysaccharide. Davis, Pittman, and ...

nificant barriers to development of high-protein commercial cultivars. The physiological and biochemical basis for increased seed protein Soybean SPC is inherited as a quantitative trait (Burconcentrations (SPC) observed in restriction-index, recurrent-selecton, 1987) and influenced by environmental effects tion breeding programs with soybean [Glycine max (L.) Merr.] are poorly understood. The ...

This review article summarized the analytical methods for the quantitative determinations of H2 receptor antagonist famotidine by spectral methods, electrochemical procedures (potentiometric titration, polarography, diferential pulse voltammetry) as well as separation procedures such as high-performance liquid chromatography (HPLC), thin layer chromatography (HPTLC) and capillary electrophoresi...