Alkaline phosphatase prepared from mammalian cell cultures was found to have alkaline inorganic pyrophosphatase activity. Both of these activities appear to be associated with a single protein, as demonstrated by: (1) concomitant purification of alkaline phosphatase and alkaline inorganic pyrophosphatase; (2) proportional precipitation of alkaline phosphatase and inorganic pyrophosphatase activ...

S ERUM ALKALINE PHOSPHATASE ACTIVITY is the enzyme analysis most frequently made in clinical laboratories. It has long been a diagnostic tool and therapeutic index in both liver and osseous disease. Its usefulness could be expanded by a method which would measure alkaline phosphatase activity rapidly, accurately, and with a minimum of technical manipulation. Ideally, the substrate for such a me...

A factor present in homogenized normal human blood leukocytes containing alka line phosphatase activity was shown to be effective in depressing the incorporation of TDR-H3 into DNA-H* when preincubated with dog bone marrow, lymph, and human chronic granulocytic leukemia cell suspensions. Human donor blood buffy coats were extracted, and an approximately 200-fold purified preparation of leukocyt...

Plasma calcium and phosphate concentrations and alkaline phosphatase activities were examined retrospectively in 50 patients with histologically proven osteomalacia and 50 age- and sex-matched control subjects with normal bone histology. An abnormal plasma alkaline phosphatase activity was more useful than an abnormal plasma calcium or phosphate concentration in distinguishing between normal an...

Leucocyte alkaline phosphatase activity has been determined in a normal group, a group of pregnant patients, and a group of patients with ectopic pregnancy.A great rise in alkaline phosphatase occurred in pregnant patients in their third trimester, but the results were only slightly raised in the ectopic group. Five of six consecutive cases gave results which fell within the normal range. The m...

RATIONALE Decreased alkaline phosphatase activity after infant cardiac surgery is associated with increased post-operative cardiovascular support requirements. In adults undergoing coronary artery bypass grafting, alkaline phosphatase infusion may reduce inflammation. Mechanisms underlying these effects have not been explored but may include decreased conversion of extracellular adenine nucleot...

Plasma alkaline phosphatase and inorganic phosphorus levels were determined for 52 nestling Spanish imperial eagles from two wild populations and 22 captive adults and subadults (10 adults and 12 subadults). The exact age was known for all birds. Mean alkaline phosphatase and inorganic phosphorus were higher in chicks than in the captive adults and subadults. Sex differences were not observed, ...

Vascular Calcification To the Editor: In their editorial on vascular calcification, Demer and Tintut1 incorrectly state that pyrophosphate reduces calcification by inhibiting alkaline phosphatase. Actually, the opposite is true. Alkaline phosphatase promotes calcification by reducing pyrophosphate levels. Pyrophosphate is a substrate for alkaline phosphatase,2 and this enzyme regulates pyrophos...

The existence of an alkaline phosphatase-lipoprotein-X complex was demonstrated as an abnormally moving band in the agar-agarose gel alkaline phosphatase pattern of sera from 58 patients in a group of 72 patients whose sera contained lipoprotein-X. The position of the abnormally moving band in the agar-agarose gel alkaline phosphatase pattern and the percentage of alkaline phosphatase activity ...