The phosphorylation of 2-deoxyglucose by respiring Krebs-2 ascites tumor cells and the effect of 2-deoxyglucose metabolism on cell orthophosphate, acid-labile phos phate, and adenine nucleotide-phosphate in these cells was investigated. In the presence of 2.0 mM orthophosphate 2-deoxyglucose (2DG) was taken up initially very rapidly, 5.7 /umoles/min/ml of cells, but uptake fell off to a steady ...

In this study, time of calcium phosphate formation on Ti6Al4V alloy with or without alkali and heat treatments was investigated. Specimens were soaked in 0, 5, 10 M solutions of NaOH at temperatures of 60 or 80 °C for 24, 72 h. Their surfaces were characterized using scanning electron microscopy and thin film X-ray diffraction. It was found that optimum condition is 72h soaking in 5 M NaOH in 8...

The radioactive compounds to be observed when algae or green leaves are allowed to photosynthesize in C1402 for short periods are almost all phosphorylated derivatives of sugars. Of these, phosphate esters of trioses, sedoheptulose, and fructose are the first to incorporate Cl4 and are followed closely by ribulose diphosphate, glucose-6-phosphate, and a phosphate of mannose. It has been noted, ...

1. Osmium tetroxide in dilute ammonia oxidizes various pyrimidine nucleosides at different rates. Thymidine is oxidized about 45 times as fast as deoxycytidine. The phosphate groups may be eliminated from oxidized thymine nucleotides by successive treatments with alkali and then with diphenylamine in aqueous formic acid. The reactions can be applied to the selective degradation of thymidine in ...

AIM To evaluate the therapeutic effect of netrin-4 on the early acute phase of inflammation in the alkali-burned eye. METHODS Eye drops containing netrin-4 or phosphate buffered saline (PBS) were administered to a alkali-burn-induced corneal acute inflammatory model four times daily. The clinical evaluations, including fluorescein staining and inflammatory index, were performed on day 1, 4 an...