This study illustrates the optimisation of the experimental conditions of three sequential steps for chondroitin sulphate (CS) recovery from three cartilaginous materials of Scyliorhinus canicula by-products. Optimum conditions of temperature and pH were first obtained for alcalase proteolysis of head cartilage (58 °C/pH 8.5/0.1% (v/w)/10 h of hydrolysis). Then, similar optimal conditions were ...

Alcalase-derived gelatin hydrolysates were glycated with glucosamine in the presence (+) or absence (-) of transglutaminase (TGase), and their antimicrobial activities toward Escherichia coli AW 1.7 were studied. Glycation treatments were subjected to concanavalin A affinity chromatography to selectively collect the glycopeptide-enriched fractions and the changes in antimicrobial activity were ...

Calcitonin gene related peptide (CGRP) related molecules were purified from sardine hydrolysates prepared using 0.1% alcalase and two hours of hydrolysis. Gel exclusion chromatography and HPLC performed purification of these molecules. The purified molecules were characterised using specific CGRP radioimmunoassays and radioreceptoraasays. From 22 mg of crude extract, we obtained 14 μg of CGRP r...

Palm (Elaeis guineensis Jacquin) kernel cake protein (PKCP) was extracted and hydrolyzed using Alcalase® 2.4L to obtain hydrolysate (PKCPH), then fractionated size exclusion chromatography. PKCPH consisted of predominantly fraction one (PKCPH1), containing two peptides (4.9 kDa 6.3 kDa) with functional amide (6.75–7.04 ppm) amine (1.5–2.0 groups. PKCP shared similar amino acid profiles. PKCPH1 ...

the objective of this study was to produce fish protein hydrolysate (fph) with the most antioxidant activity, by enzymatic hydrolysis from (carassius carassius). the protein of crucian carp a low value fish was hydrolyzed by alcalase 2.4l and the optimal hydrolysis parameters of strongest antioxidant capacity of peptides were obtained using response surface methodology (rsm) by the central rota...

Fish proteins are found in the flesh, head, frames, fin, tail, skin and guts of the fish in varying quantities. Unutilized fish and fish processing waste can be used to produce fish proteins which contain amino acids and many bioactive peptides. After removing the fish flesh during the fish processing operation, all other parts are considered wastes which are not properly utilized. The aim of t...

Controlled enzymatic modification proteins are currently being used as good sources of bioactive protein ingredients, and hydrolysates derived from bighead carp muscles may serve as antioxidants through the control of the processing-related parameters. The antioxidant ability was evaluated with regard to the scavenging effect on free radical DPPH, OH and O2 . Due to the robustness, fault tolera...

Regularly consuming peanuts has been reported to have many health benefits. Peanut flour, a by-product of peanut oil processing, higher protein and dietary fiber contents than kernels, but its application as source in foods supplement is limited due the fear allergy. This study indicates that hydrolysis flour (12% lipid) up 4 h with Alcalase lowered immunoreactivity both soluble insoluble porti...