THE SEPARATION of albumin from its associated globulins in serum or plasma has exercised physiologists and biochemists for many years. Several methods are available for assessing the albumin content of serum by prior precipitation of globulins (1-9). In 1949 Martin and Morris (10) examined a series of methods (1, 2, 5-7) and concluded, by comparison with the results of “free” electrophoresis, t...

In 1885-6 Kauder observed that globulin was precipitated from serum when the concentration of ammonium sulphate reached 24 to 29 per cent of full saturation and that its precipitation was complete when the concentration reached 36 to 46 per cent of complete saturation. Thereafter no further precipitation took place until the concentration reached 64 per cent of full saturation, when albu-min st...

The isolation procedure of the seed proteins of Amaranthus blitum have been analyzed at different pH conditions. Qualitative studies were carried out by using electrophoretic technique sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). Mainly four protein fractions i.e. albumin, globulin, prolamin and glutelin were obtained. Protein isolates were prepared by (a) extraction a...

Introduction: Psoriasis has been considered as a challenging dermatological disease with various systemic events. Multiple factors have been implicated in the pathogenesis of psoriasis backed up by numerous studies. It is associated with many biochemical abnormalities which have been implicated in the pathogenesis of the disease. Objectives: The purpose of this study was to compare the levels o...

Chemical and electrophoretic analyses of the serum proteins have become routine diagnostic investigations in many hospital laboratories. The albumin/globulin ratio decreases in a variety of disease conditions, including liver diseases, the nephrotic syndrome, protein malnutrition, myeloma, etc. It is therefore very important that the normal variations in the albumin-globulin ratio should be wel...

The serum protein pattern of Africans is usually found to differ from that of Europeans, being characterized by lower albumin levels and higher total globulin levels. There is evidence that this may be due to a number of possibly interrelated factors, viz. parasitism, malnutrition, liver disease, and, to a limited extent, genetic considerations. There is no unambiguous evidence that diet plays ...

It is generally accepted that complement may be split into a mid-piece and an end-piece. The mid-piece is thought to be in the globulin fraction, and the end-piece in the albumin fraction. The restoration of complement activity by putting together the albumin and globulin fractions does not prove, however, that each fraction contained a part of the complement, for the albumin fraction can be re...