Hybrid, bivalent antibody molecules bearing specific combining sites for both ovalbumin and bovine gamma globulin were produced by reoxidation of a mixture of the 3.5S fragments of the two specifically purified antibodies. The dual specificity and the properties of the hybrid antibody were demonstrated by mixed agglutination and two stage agglutination experiments, and by test systems utilizing...

Of the first 128 patients to receive either heart or heart and lung transplants at Papworth Hospital, four developed Toxoplasma gondii infections acquired from the donor heart and two died. Six patients had passively acquired T gondii antibody as a result of blood transfusions around the time of transplantation. Eight patients developed antibodies against T gondii, which were detectable by chan...

We have developed a one-point microcapsule agglutination test (MCAT) for the serodiagnosis of leptospirosis. The MCAT kit was evaluated for use in humans by six WHO Collaborating Centres for Reference and Research on Leptospirosis. The laboratories classified their serum samples on the basis of the microscopic agglutination test (MAT) and the following screening tests: enzyme-linked immunosorbe...

HERR, S., TE BRUGGE, LESLEY A. & GUINEY, M. C. M., 1982. The value of the microtitre serum agglutination test as a second screening test in bovine brucellosis. Oruierstepoort Journal of Veterinary Research, 49, 23--28 (1982). The use of the serum agglutination test (SAT) as a 2nd screening test led to a reduction in false negative screening reactions especially in sera delayed in transit and pr...

A latex agglutination test for determination of antibody against cytomegalovirus was compared with five other methods: a solid-phase fluorescent immunoassay, an indirect hemagglutination test, two solid-phase enzyme immunoassays, and an indirect fluorescent-antibody method, with sera collected from 210 random blood donors. Of the sera tested, 28% were positive for anti-cytomegalovirus by concor...

The serum of patients with rheumatoid arthritis contains a factor which causes agglutination of sensitized particulate bodies. This phenomenon has been demonstrated using sheep red cells (Waaler, 1940), collodion particles (Wallis, 1946), bacteria (Wager, 1950), polysterene latex particles (Singer and Plotz, 1956), and bentonite (Bozicevich, Bunim, Freund, and Ward, 1958). Rose, Ragan, Pearce, ...

Hemagglutination inhibition, enzyme-linked immunosorbent assay (ELISA), and passive latex agglutination were used to test 495 human serum samples for determination of rubella immunity. Overall agreements for immune status were as follows: hemagglutination inhibition versus ELISA, 94.7% (469 of 495); hemagglutination inhibition versus passive latex agglutination (1:10 dilution), 99.2% (491 of 49...

A group C latex agglutination reagent, included as the negative control in the PathoDx Strep A latex agglutination test (Diagnostic Products Corp., Los Angeles, Calif), was compared with culture for rapid detection of group C streptococci (Streptococcus equi, S. equisimilis, S. zooepidemicus, and S. dysgalactiae) from swabs of veterinary specimens. The overall sensitivity of the latex test was ...

BACKGROUND: Leptospirosis is a worldwide zoonosis causedby Leptospira interrogans. Leptospirosis results in decreasedmilk production, abortion, stillbirth, infertility and mortality,which causes financial loss in the cattle industry. OBJECTIVES:The aim of this research was to perform a serological andbacteriological study of leptospirosis in 6 industrial dairy herdsand 3 feedlots with previous ...