Human factor VIII has been isolated from a high purity factor VIII concentrate by immunoaffinity chromatography and HPLC on Mono Q gel. Two fractions of factor VIII were obtained with a specific activity of approximately equal to 7000 units/mg. The major fraction contained eight peptide chains of 200, 180, 160, 150, 135, 130, 115, and 105 kDa plus one doublet chain of 80 kDa. The minor fraction...

In the present study, sheep crude hydatid cyst fluid antigen (SCHCFA) was prepared by ammonium sulfate precipitation, centrifugation and dialysis. SDS-PAGE of SCHCFA showed seven polypeptides of 72.8 kDa, 66.5 kDa, 60.2 kDa, 41 kDa, 25.1 kDa, 19 kDa and 8 kDa when stained with Coomassie briliant Blue R-250. The initial part of the ascending loop of the first peak (PI) when resolved by gel filtr...

AIM To identify proteins induced by androgen in Sertoli cells during spermatogenesis. METHODS We analyzed protein profiles in TM4 Sertoli cells treated with dihydrotestosterone (DHT) using surface enhanced laser desorption ionization time-of-flight mass spectrometry (SELDI-TOF-MS). RESULTS We found increases in the expression of a 5.0-kDa protein at 15 min, an 11.3-kDa protein at 24 h and 4...

Treatment of human cervical epithelial CaSki cells with ATP or with the diacylglyceride sn-1,2-dioctanoyl diglyceride (diC8) induced a staurosporine-sensitive transient increase, followed by a late decrease, in tight-junctional resistance (R(TJ)). CaSki cells express two immunoreactive forms of occludin, 65 and 50 kDa. Treatments with ATP and diC8 decreased the density of the 65-kDa form and in...

The physico-mechanical properties of fructan fractions in different agave species without Tequila Denomination Origin (DOT) need to be studied identify and expand industrial applications. rheological physicochemical characterization three Agave tequilana cv. cenizo fructans DOT, with degree polymerization (DP) was carried out propose their use as an additive the food industry. Fractions were ob...

Probiotic and glutamine supplementation increases tissue Hsp72, but their influence on extracellular Hsp72 (eHsp72) has not been investigated. The aim of this study was to investigate the effect of chronic probiotic supplementation, with or without glutamine, on eHsp72 concentration before and after an ultramarathon. Thirty-two participants were split into 3 independent groups, where they inges...

In this study, the effects of four different temperatures on sporulation and development 6 Bacillus species, 2 native 4 reference were investigated. The SDS PAGE analysis emerged that two proteins, 40 39 kilodalton (kDa), produced by sphaericus ATCC 2362 after 24 48, respectively hours at 48°C, local isolate 48 42°C 31 48°C. Additionally firmus (ATCC 14573) a kDa protein 52°C thuringiensis var....

The interest in separating and concentrating bioactives minerals from seaweeds extracts is growing due to large health benefits of these substances. aim this study was investigate the separation components Fucus vesiculosus seaweed by tangential ultrafiltration apply Hermia’s models adapted crossflow understand fouling mechanism. influence membrane cut-off varying 5 150 kDa, velocity 0.081 0.09...