Experiments were conducted to demonstrate the role of male Aedes aegypti mosquitoes in the maintenance and transmission of chikungunya virus (CHIKV) to female mosquitoes. We demonstrated that infected male mosquitoes are capable of infecting females during mating. The infection rate in female mosquitoes was 11% when virgin female mosquitoes were allowed to coinhabit with infected males. The bod...

Studies were conducted to determine the ability of Aedes excrucians (Walker), Ae. cinereus Meigen, and Ae. communis (De Geer) group mosquitoes, collected in an Ockelbo (OCK) virus enzootic area in central Sweden, to transmit this virus. All three species were highly susceptible to infection; at least 96% of the specimens of each species became infected after ingesting blood from a viremic chick...

Nineteen species of larval mosquitoes were collected from 209 sites in 47 of West Virginia's 55 counties over a 7-month period (April-October) in 1992. Aedes abserratus is recorded from the state for the first time.

The genus Flavivirus includes a range of mosquito-specific viruses in addition to well-known medically important arboviruses. Isolation and comprehensive genomic analyses mosquitoes collected Bolivia resulted the identification three novel flavivirus species. Psorophora (PSFV) was isolated from albigenu . coding sequence PSFV polyprotein shares 60 % identity with that Aedes -associated lineage ...

Putative epidemic/epizootic eastern equine encephalomyelitis virus (EEE) vector populations were compared at 15 recent (1982-90) human and horse case sites in Bristol and Plymouth counties in southeastern Massachusetts. Carbon dioxide-baited American Biophysics Corporation light traps were used for trapping adult mosquitoes to estimate biting risk in these foci of known transmission. Population...

Tires that were either crudely chopped or more finely processed into shreds contained viable eggs. Field-collected remnants of 2-3 chopped tires contained viable Aedes albopictus eggs. After shredding tires seeded with mosquito eggs, 34 (4.6%) of an estimated 746 Ae. albopictus eggs and 21 (2.7%) of an estimated 774 Aedes triseriatus eggs survived. Chopped and shredded tire remnants may serve a...

The primary vector at the origin of the 2007 outbreak in Libreville, Gabon is identified as Aedes albopictus, trapped around the nearby French military camp. The Chikungunya virus was isolated from mosquitoes and found to be identical to the A226V circulating human strain. This is the first field study showing the role of the recently arrived species Aedes albopictus in Chikungunya virus transm...

We investigated the susceptibility of an Italian population of Culex pipiens mosquitoes to Zika virus (ZIKV) infection, tested in parallel with Aedes aegypti, as a positive control. We analysed mosquitoes at 0, 3, 7, 10, 14, 20 and 24 days after an infectious blood meal. Viral RNA was detected in the body of Cx. pipiens up to three days post-infection, but not at later time points. Our results ...

Zika virus transmission is primarily vector-borne, mainly via Aedes mosquitoes. The virus can also be found in various body fluids and transmission by sexual contact has also been reported. Aedes aegypti, the principal vector, is found in tropical and subtropical climates and adapts well to urban areas, living in and around human habitations. The areas affected by Zika virus lie within areas af...

In this video, Nijole Jasinskiene demonstrates the methodology employed to generate transgenic Aedes aegypti mosquitoes, which are vectors for dengue fever. The techniques for correctly preparing microinjection needles, desiccating embryos, and performing microinjection are demonstrated.