A rapid, manyfold increase in phosphorylation of cardiac microsomes consisting primarily of sarcoplasmic reticulum was seen when these membranes were incubated in the presence of a bovine cardiac adenosine 3’:5’-monophosphate (cyclic AMP)-dependent protein kinase (protein kinase) and cyclic AMP. Over 85 % of the 3*P associated with membrane protein under similar conditions was identified cs pho...

The factors that limit the stability of adenylate kinase and acetate kinase in solution have been examined and compared with those that determine stability under conditions encountered during photochemically initiated polymer gel formation in solutions of acrylamide and N,N'-methylene-bisacrylamide. Both adenylate kinase (from rabbit and pig muscle) and acetate kinase (from E. Coli) contain cys...

A rapid, manyfold increase in phosphorylation of cardiac microsomes consisting primarily of sarcoplasmic reticulum was seen when these membranes were incubated in the presence of a bovine cardiac adenosine 3’:5’-monophosphate (cyclic AMP)-dependent protein kinase (protein kinase) and cyclic AMP. Over 85 % of the 3*P associated with membrane protein under similar conditions was identified cs pho...

Of the uncloned ODA genes required for outer dynein arm assembly in Chlamydomonas, ODA5 and ODA10 are of particular interest because they do not encode known subunits of the outer arm or the outer dynein arm-docking complex (ODA-DC), and because genetic studies suggest their products interact. Beginning with a tagged oda5 allele, we isolated genomic and cDNA clones of the wild-type gene. ODA5 p...

Members of the AMP-activated protein kinase (AMPK) family are activated by phosphorylation at a conserved threonine residue in the activation loop of the kinase domain. Mammalian AMPK adopts a phosphatase-resistant conformation that is stabilized by binding low energy adenylate molecules. Similarly, binding of ADP to the Snf1 complex, yeast AMPK, protects the kinase from dephosphorylation. Here...

The role of AMP in photophosphorylation was studied using rapid mixing acid quench techniques. Fragmented spinach chloroplast membranes or subchloroplast particles were illuminated and rapidly mixed with [32P]orthophosphate and AMP at pH 7 for 10 ms to 60 s after which time perchloric acid was added to quench the reaction. ATP was found to be the primary and predominant nucleotide labeled. It w...

Several biochemical markers in the cerebrospinal fluid (CSF) of 120 patients with serous meningoencephalitis (SM) of viral origin were compared with those of 74 patients with viral or bacterial infections accompanied by neck stiffness but no CSF abnormality (i.e., meningism). CSF adenylate kinase was higher (P less than 0.025) in SM and correlated with lactate concentration (r = 0.37; P less th...

B-CLL is the most frequent type of leukemia in the Western countries. The disease, common among the elderly, follows a variable course in terms of survival time and symptoms. There is evidence that the accumulation of lymphocytes in peripheral blood and bone marrow is due to a cell resistance to apoptosis rather than to highly proliferative cells. Genetic mechanisms that lead to the development...

All known nucleoside monophosphate kinases contain an invariant sequence Asp-Gly-Phe(Tyr)-Pro-Arg. In order to understand better the structural and functional role of individual amino acid residues belonging to the above sequence, three mutants of Escherichia coli adenylate kinase (D84H, G85V, and F86L) were produced by site-directed mutagenesis. Circular dichroism spectra revealed that the sec...