Myzus persicae is a devastating pest affecting potato production. Intron-containing hairpin RNA (ihpRNA) silenced the odorant-binding protein 8 (OBP8) for enhanced protection against in potatoes. OBP8 was isolated from M. persicae, sequenced, with allotment of GenBank ID. ERNAi used to design siRNA targets no off-targets. Multiple Sequence Alignment show resemblance Acyrthosiphon pisum, Rhopalo...

Many insects are associated with obligate symbiotic bacteria, which are localized in specialized cells called bacteriocytes, vertically transmitted through host generations via ovarial passage, and essential for growth and reproduction of their hosts. Although vertical transmission is pivotal for maintenance of such intimate host-symbiont associations, molecular and cellular mechanisms underlyi...

The western chinch bug, Blissus occiduus Barber, is a serious pest of buffalograss, Buchloe dactyloides (Nuttall) due to physical and chemical damage caused during the feeding process. Although previous work has investigated the feeding behaviors of chinch bugs in the Blissus complex, no study to date has explored salivary gland morphology and the associated salivary complex of this insect. Who...

Insect cuticular hydrocarbons (CHCs) play critical roles in reducing water loss and chemical communication. Species-specific CHC profiles have been used increasingly as an excellent character for species classification. However, considerably less is known about their potential for population delimitation within species. The aims of this study were to develop a solid-phase microextraction (SPME)...

Flavonoids play an important role in interactions of plants with the environment. Liquid chromatography (HPLC) was used to determine the flavonoid profiles (especially luteolin, tricin, and chrysoeriol glycosides), their total concentrations, and changes in the amounts of eight flavones found in the aerial parts of alfalfa (Medicago sativa L.) (Fabaceae) Radius cv., uninfested and infested by t...

It has been claimed that plant architecture can alter aphid reproductive rates, but the mechanism driving this effect has not been identified. We studied interactions between plant architecture, aphid density, environmental conditions, and nutrient availability on the reproduction of pea aphids [Acyrthosiphon pisum (Harris)] using four near-isogenic peas (Pisum sativum L.) that differ in morpho...

Supplemental Experimental Procedures Aphids Strain ISO, a parthenogenetic clone of the pea aphid Acyrthosiphon pisum was used in this study. The insects were reared on Vicia faba at 15°C in a long-day regime of 16 h light and 8 h dark. Parthenogenetic apterous adults (12 to 15 days old) were used for the experiments. The antibody against A. pisum RlpA4 was raised in rabbits using recombinant pr...