Actinobacillus (A.) pleuropneumoniae is a causative agent of porcine pleuropneumonia, which has been reported in most countries. A genomic expression library of A. pleuropneumonia serotype 7 was constructed to identify potential vaccine candidates. Plasmid DNA from the sub-libraries (L1 to L10) was used to immunize BALB/c mice. Then, 2 weeks after the final immunization, the groups of mice were...

Bactericidal activity of human -defensin 3 (hBD-3) against Streptococcus mutans and Actinobacillus actinomycetemcomitans was inhibited in a dose-dependent manner by the presence of saliva and/or serum. Increasing the concentration of hBD-3 partially overcame this inhibition. A fast bactericidal effect was observed against both bacterial strains, suggesting a potential therapeutic use for hBD-3 ...

As a potential intermediary feedstock, succinic acid takes an important place in bulk chemical productions. For the first time, a method combining Plackett-Burman design (PBD), steepest ascent method (SA), and Box-Behnken design (BBD) was developed to optimize Actinobacillus succinogenes ATCC 55618 fermentation medium. First, glucose, yeast extract, and MgCO(3) were identified to be key medium ...

The complete genome of Nitrosomonas ureae strain Nm10, a mesophilic betaproteobacterial ammonia oxidizer isolated from Mediterranean soils in Sardinia, Italy, is reported here. This genome represents a cluster 6a nitrosomonad.

In this paper, we describe our synthesis of four key building blocks for the total synthesis of psymberin (1) and its C4 epimer (2). Despite early difficulties in processing material to the advanced intermediate stage, we have been successful in developing high-yielding syntheses for the pyran core, natural side chain, 4-epi side chain, and aryl fragments of the molecule. Our findings from the ...

This case study describes sudden deaths of pigs in a North Carolina production system at three different finishing facilities between June 2019 and February 2020. The cases involved 3- to 6-month-old the same genetics but from sow farm flows. Pigs all sites had gross lesions that included firm, deep red purple lungs. Based on laboratory results tissue samples, were diagnosed with Actinobacillus...

The first total synthesis of Aeruginazole A, prepared via a convergent strategy that involved both solid-phase peptide synthesis and solution phase chemistry and that enabled conservation of the stereochemistry of the intermediates, is reported.

An efficient synthesis of the polypropionate framework of callystatin A has been achieved by utilizing the Shimizu reaction in an iterative fashion.