Mechanical forces have long been studied in relation to stem cell differentiation or cell migration, however, their significance during brain and neural circuit formation are poorly understood. We present a magnetic gradient platform that allows investigation of small scale forces acting on arrays of neurons in parallel. The approach draws magnetic nanoparticles within neurons towards patterned...

Rho-dependent transcription terminators participate in sophisticated genetic regulatory mechanisms, in both bacteria and phages; they occur in regulatory regions preceding the coding sequences of genes and within coding sequences, as well as at the end of transcriptional units, to prevent readthrough transcription. Most Rho-dependent terminators have been found in enteric bacteria, but they als...

Eukaryotic gene expression is precisely regulated at all points between transcription and translation. In this review, we focus on translational control mediated by the 3'-untranslated regions (UTRs) of mRNAs. mRNA 3'-UTRs contain cis-acting elements that function in the regulation of protein translation or mRNA decay. Each RNA binding protein that binds to these cis-acting elements regulates m...

The SM50 gene encodes a minor matrix protein of the sea urchin embryo spicule. We carried out a detailed functional analysis of a cis-regulatory region of this gene, extending 440 bp upstream and 120 bp downstream of the transcription start site, that had been shown earlier to confer accurate skeletogenic expression of an injected expression vector. The distal portion of this fragment contains ...

 Summary I.  358 II.      359 1. Seed development 359 (a) Maturation 359 (b) Dormancy and germination 361 2. Vegetative responses 365 (a) Root growth 366 (b) Overlapping stress response pathways 367 III. ‘  ’      371 1. ABA-regulated gene products 371 2. Cis-acting elements 372 3. Trans-acting fa...

RNA localization is a widely utilized strategy employed by cells to spatially restrict protein function. In Saccharomyces cerevisiae asymmetric sorting of mRNA to the bud has been reported for at least 24 mRNAs. The mechanism by which the mRNAs are trafficked to the bud, illustrated by ASH1 mRNA, involves recognition of cis-acting localization elements present in the mRNA by the RNA-binding pro...

Nucleosome positioning on the chromatin strand plays a critical role in regulating accessibility of DNA to transcription factors and chromatin modifying enzymes. Hence, detailed information on nucleosome depletion or movement at cis-acting regulatory elements has the potential to identify predicted binding sites for trans-acting factors. Using a novel method based on enrichment of mononucleosom...

The mating-type genes of Schizosaccharomyces pombe are found at three locations in the same chromosomal region. These genes are in an active configuration at the mat1 locus and in an inactive configuration at the mat2 and mat3 loci. The mechanism that represses transcription of mat2 and mat3 also inactivates other promoters introduced nearby and is accompanied by a block to meiotic recombinatio...

If T t = e Zt is a positive one-parameter contraction semigroup acting on l p (X) where X is a countable set and 1 ≤ p < ∞, then the peripheral point spectrum P of Z cannot contain any non-zero elements. The same holds for Feller semigroups acting on L p (X) if X is locally compact.

If Tt = eZt is a positive one-parameter contraction semigroup acting on lp(X) where X is a countable set and 1 ≤ p < ∞, then the peripheral point spectrum P of Z cannot contain any non-zero elements. The same holds for Feller semigroups acting on Lp(X) if X is locally compact.