Crystal violet exhibited characteristics of an uncoupler of oxidative phosphorylation, i.e. it released respiratory control, hindered ATP synthesis, enhanced ATPase activity, and produced swelling of isolated rat liver mitochondria. Maximal stimulation of respiration, ATPase activity, and swelling was observed at a concentration of 40 microM. The inhibition of State 3 respiration by oligomycin ...

Stadler, L. J., "The Comparison of Ultra-Violet and X-ray Effects on Mutation," Cold Spring Harbor Symp. Quant. Biol., 9, 188-177 (1941). Stadler, L. J., and Uber, Fred M., "Genetic Effects of Ultra-Violet Radiation in Maize. IV. Comparison of Monochromata Radiations," Genetics, 27, 84-118 (1942). Stadler, L. J., and Roman, Herschel, "The Effect of X-rays upon Mutation of the Gene A in Maize," ...

Many fish, amphibians, reptiles, birds, and some mammals use UV vision for such basic activities as foraging, mate selection, and communication. UV vision is mediated by UV pigments in the short wavelength-sensitive type 1 (SWS1) group that absorb light maximally (lambda max) at approximately 360 nm. Reconstructed SWS1 pigments of most vertebrate ancestors have lambda max values of approximatel...

The adsorption of Acid blue 25, basic blue 9, basic violet 3, Pb(2+), Ni(2+), Zn(2+) and Cd(2+) ions has been studied in single and dye-metal binary solutions using two mineral materials: Clinoptilolite (CL) and ER (Erionite). These zeolites were characterized by FT-IR spectroscopy; potentiometric titration and nitrogen adsorption isotherms at 77 K to obtain their textural parameters. Results i...

The results 1 from measurements made with the glass electrode and spectrophotometer show that brilliant cresyl blue penetrates as free base into the vacuoles of living cells of Nitella. The present paper describes similar experiments with azure B, methylene blue, and crystal violet. Since the methods for the condition of cells, extraction of the sap, penetration experiments, extraction of free ...

Acrylamide/itaconic acid (AAm/IA) hydrogels containing different quantities of itaconic acid have been irradiated with g radiation. The hydrogels were used in an experiment concerning the adsorption of cationic dyes such as Basic Blue 9, Basic Violet 1 and Basic Blue 12. In the experiments of the adsorption of dyes from their synthetic aqueous solutions, type S adsorption isotherms were found. ...

BACKGROUND Quantification of free monoclonal light chains in urine [Bence Jones proteins (BJPs)] is used to diagnose multiple myeloma and to evaluate response to treatment. We have developed and evaluated an optimized approach for quantification of BJPs. METHODS High-resolution gel electrophoresis of unconcentrated urine and albumin calibrators was carried out on Sebia's Hydrasys instrument w...

In a previous communication 1 a selective bactericidal p roper ty possessed by gentian violet was described, and observations were reported to indicate the nature of this chemical affinity as exhibited in a study of a large number of representative bacteria. It was shown that bacteria could be sharply divided into two groups by their behavior toward gentian violet, either by staining the organi...

A rapid technique for the immunological identification of group B streptococci in vaginal swabs is reported. Vaginal swabs obtained from 567 pregnant women at term or during labor were incubated for eight hrs in Todd Hewitt broth containing 15 micrograms per ml nalidixic acid, one microgram per ml polymixin, and 0.1 microgram per ml crystal violet (NPC broth). After streaking the swabs on blood...

PROCEDURES The following have been used with success: haematoxylin and eosin, Perls's reaction for ferric iron, the periodic acid-Schiff reaction for carbohydrates, the alcian blue method for acid mucopolysaccharides, the Feulgen reaction for desoxyribonucleic acid, the periodic acid-hexamine silver method, Luxol fast blue for myelin, solochrome cyanine as a general stain, Heidenhain's iron hae...