Poor laboratory equipment and few human resources have made it difficult to implement microscopic diagnosis of pulmonary tuberculosis (TB) on a large scale basis worldwide. Three hundred sputum samples from patients in Cameroon were studied by using the CyScope®, a new light-emitting, diode-based, fluorescence microscope, to compare auramine-rhodamine fluorescence with the conventional Ziehl-Ne...

Calf thymus histories comprising two fractions, one rich in lysine, the other having roughly equal amounts of lysine and arginine, Loligo testes histones rich in arginine, and salmine, are compared with respect to their amino acid compositions, and their staining properties when the proteins are fixed on filter paper. The three types of basic proteins; somatic, arginine-rich spermatid histones,...

In India, pulmonary tuberculosis accounts for 85% of cases and extrapulmonary tuberculosis accounts for 15% of cases with bone and joint involvement is seen in 1 to 3% of cases. Primary tuberculous osteomyelitis of the sternum is a rare form of extrapulmonary tuberculosis. A 51-year-old male presented with 10 years history of swelling over the anterior chest wall and pain for past 6 months. Fin...

Nontuberculous mycobacteria (NTM) are rarely isolated from peritoneal dialysis (PD)-associated catheter infections. However, NTM infection is usually difficult to treat and leads to catheter loss. Prompt diagnosis is essential for appropriate treatment. A 70-year-old Japanese man who had been on PD for 2 years and with a medical history of 2 episodes of exit site infections (ESIs) due to methic...

Background and Objective: Tuberculosis is still a major health problem, involving about 1/3 of the world ́s population. Diagnosis is difficult when we only use Ziehl-Neelson staining. Many cases may be missed. A more rapid and sensitive diagnostic method is necessary. PCR may be helpful. The aim of this study is to compare PCR, Zieh-Neelsen staining and histopathologic findings in diagnosis of t...

cryptosporidium is a globally distributed protozoan parasite and one of the most common causes of infection and diarrhea in humans and cattle. the aim of the present study was to determine the species of cryptosporidium among cattle with diarrhea by a nested pcr-rflp technique at cryptosporidium oocyst wall protein ( cowp ). fecal samples from 158 calves aged 1-20 weeks were collected from 10 d...

cystoisospora belli, formerly isospora belli , as an opportunistic infection agent, is seen in immunocompromised patients like htlv-1. we describe here cystoiso­sporiasis in an htlv1 iranian female in mashhad, northwestern iran in 2012 who presented with a debilitating diarrheal illness and great weight loss. c. belli was de­tected in her stool by modified acid-fast staining and then by molecul...

ABSTRACT Mycobacteriosis was detected in seven out of one Atlantic mackerel (Scomber scombrus) that purchased for human consumption from a fish market. The apparently healthy but during cleaning, several granulomatous foci were noticed the visceral organs. Histopathological examination lesions revealed numerous characterized by caseous necrosis center lesion surrounded epithelioid giant cells. ...