A particulate enzyme preparation from the fungus imperfectus C~yptococcus laurentii var. flavescens (NNRL Y-1401) catalyzes the transfer of galactosyl residues from UDP-[WIgalactose to an endogenous acceptor. The radioactive reaction product is particle-bound, but is solubilized by pronase treatment. The solubilized radioactive material migrates as a single peak on a column of Sepharose 4-B, in...

2-Aminoethylarsonic acid was tested for its ability to act as a substrate for ethanolamine-phosphate cytidylytransferase as a cytidylyl acceptor in place of ethanolamine phosphate. The expected product, like all mixed anhydrides of arsonic acids, should hydrolyse spontaneously with regeneration of the substrate analogue and CMP formation; such CMP production was observed. The limiting velocity ...

We identify an Arabidopsis pyridoxal-phosphate-dependent aminotransferase, VAS1, whose loss-of-function simultaneously increases amounts of the phytohormone auxin and the ethylene precursor 1-aminocyclopropane-1-carboxylate. VAS1 uses the ethylene biosynthetic intermediate methionine as an amino donor and the auxin biosynthetic intermediate indole-3-pyruvic acid as an amino acceptor to produce ...

Schiffbase was obtained by condensation of camphor with 4-amino-phenol .The charge transfer complex of the Schiff base as a donor molecule with picric acid as acceptor molecule in methanol were estimated. Schiff-base compound and charge transfer complex were identified by elemental analysis, infrared spectra and UV-visible spectra.The physical parameters of the CT complex were calculated by app...

In the title compound, C(10)H(9)ClN(2)O(3)·H(2)O, the water mol-ecule is a hydrogen-bond donor to the amide and carbonyl O atoms of two acid mol-ecules; it is also a hydrogen-bond acceptor to the acid OH group and the amide H atom. The hydrogen-bonding inter-actions give rise to a two-dimensional array.

The asymmetric unit of the title compound, C21H14O6, com-prises two symmetrically independent mol-ecules that form a locally centrosymmetric hydrogen-bonded dimer, with the planes of the corresponding carb-oxy-lic acid groups rotated by 15.8 (1) and 17.5 (1)° relative to those of the adjacent benzene rings. The crystal as a whole, however, exhibits a noncentrosymmetric packing, described by the...

The acceptor substrate specificity of UDP-GalNAc:polypeptide N-acetylgalactosaminyltransferase (GalNAc-transferase) was inferred from the amino acid sequences surrounding 196 O-glycosylation sites extracted from the National Biomedical Research Foundation Protein Database. When analyzed according to the cumulative enzyme specificity model (Poorman, R.A., Tomasselli, A.G., Heinrikson, R.L., and ...

Cytidine diphosphate monoacylglycerol was prepared by the action of snake venom phospholipase on cytidine diphosphate diacylglycerol and shown to be readily acylated by rat liver microsomes. Acylation was monitored by spectrophotometric assay or by measuring the incorporation of radioactivity from ‘%-labeled acyl-CoA esters. The product was identified as CDP-diacylglycerol. The acylation reacti...

The regulon of the sigma factor RpoS was defined in Geobacter sulfurreducens by using a combination of DNA microarray expression profiles and proteomics. An rpoS mutant was examined under steady-state conditions with acetate as an electron donor and fumarate as an electron acceptor and with additional transcriptional profiling using Fe(III) as an electron acceptor. Expression analysis revealed ...